![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
RESEARCH COMMUNICATION
Faculty of Life Sciences, Bar-Ilan University, Ramat-Gan 52900, Israel
It is widely accepted that spermatozoa are translationally silent. The present study demonstrates, for the first time, incorporation of labeled amino acids into polypeptides during sperm capacitation, which was completely inhibited by mitochondrial translation inhibitors but not by the cytoplasmic translation inhibitor. Unlike 80S cytoplasmic ribosomes, 55S mitochondrial ribosomes were present in polysomal fractions, indicating that these ribosomes are actively involved in protein translation in spermatozoa. Inhibition of protein translation significantly reduced sperm motility, capacitation and in vitro fertilization rate. Thus, contrary to the accepted dogma, nuclear genes are expressed as proteins in sperm during their residence in the female reproductive tract until fertilization.
[Keywords: Sperm; protein translation; capacitation; fertilization; mitochondrial ribosomes]
Received September 20, 2005; revised version accepted December 19, 2005.
Article published online ahead of print. Article and publication date are at http://www.genesdev.org/cgi/doi/10.1101/gad.367606.
E-MAIL breith{at}mail.biu.ac.il; FAX 972-3-5344766.
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  T. Almog, S. Lazar, N. Reiss, N. Etkovitz, E. Milch, N. Rahamim, M. Dobkin-Bekman, R. Rotem, M. Kalina, J. Ramon, et al. Identification of Extracellular Signal-regulated Kinase 1/2 and p38 MAPK as Regulators of Human Sperm Motility and Acrosome Reaction and as Predictors of Poor Spermatozoan Quality J. Biol. Chem., May 23, 2008; 283(21): 14479 - 14489. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. A. Mitchell, B. Nixon, M. A. Baker, and R. J. Aitken Investigation of the role of SRC in capacitation-associated tyrosine phosphorylation of human spermatozoa Mol. Hum. Reprod., April 1, 2008; 14(4): 235 - 243. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Immler Sperm competition and sperm cooperation: the potential role of diploid and haploid expression Reproduction, March 1, 2008; 135(3): 275 - 283. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L.A. Mitchell, B. Nixon, and R.J. Aitken Analysis of chaperone proteins associated with human spermatozoa during capacitation Mol. Hum. Reprod., September 1, 2007; 13(9): 605 - 613. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Harris, B. Marquez, S. Suarez, and J. Schimenti Sperm Motility Defects and Infertility in Male Mice with a Mutation in Nsun7, a Member of the Sun Domain-Containing Family of Putative RNA Methyltransferases Biol Reprod, August 1, 2007; 77(2): 376 - 382. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Etkovitz, S. Rubinstein, L. Daniel, and H. Breitbart Role of PI3-Kinase and PI4-Kinase in Actin Polymerization During Bovine Sperm Capacitation Biol Reprod, August 1, 2007; 77(2): 263 - 273. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. Gilbert, N. Bissonnette, G. Boissonneault, M. Vallee, and C. Robert A molecular analysis of the population of mRNA in bovine spermatozoa Reproduction, June 1, 2007; 133(6): 1073 - 1086. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C.G. Ravina, M. Seda, F.M. Pinto, A. Orea, M. Fernandez-Sanchez, C.O. Pintado, and M.L. Candenas A role for tachykinins in the regulation of human sperm motility Hum. Reprod., June 1, 2007; 22(6): 1617 - 1625. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H.-G. Li, X.-F. Ding, A.-H. Liao, X.-B. Kong, and C.-L. Xiong Expression of CatSper family transcripts in the mouse testis during post-natal development and human ejaculated spermatozoa: relationship to sperm motility Mol. Hum. Reprod., May 1, 2007; 13(5): 299 - 306. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. E. Platts, D. J. Dix, H. E. Chemes, K. E. Thompson, R. Goodrich, J. C. Rockett, V. Y. Rawe, S. Quintana, M. P. Diamond, L. F. Strader, et al. Success and failure in human spermatogenesis as revealed by teratozoospermic RNAs Hum. Mol. Genet., April 1, 2007; 16(7): 763 - 773. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Lefievre, K. Bedu-Addo, S. J Conner, G. S M Machado-Oliveira, Y. Chen, J. C Kirkman-Brown, M. A Afnan, S. J Publicover, W C. L Ford, and C. L R Barratt Counting sperm does not add up any more: time for a new equation? Reproduction, April 1, 2007; 133(4): 675 - 684. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Liu, J. M. Brockman, B. Dass, L. N. Hutchins, P. Singh, J. R. McCarrey, C. C. MacDonald, and J. H. Graber Systematic variation in mRNA 3'-processing signals during mouse spermatogenesis Nucleic Acids Res., January 12, 2007; 35(1): 234 - 246. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Miller and G.C. Ostermeier Towards a better understanding of RNA carriage by ejaculate spermatozoa Hum. Reprod. Update, November 1, 2006; 12(6): 757 - 767. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. A. Baker, L. Hetherington, and R. J. Aitken Identification of SRC as a key PKA-stimulated tyrosine kinase involved in the capacitation-associated hyperactivation of murine spermatozoa J. Cell Sci., August 1, 2006; 119(15): 3182 - 3192. [Abstract] [Full Text] [PDF]
|
|
