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GENES & DEVELOPMENT 21:3319-3330, 2007
©2007 by Cold Spring Harbor Laboratory Press; ISSN 0890-9369/ $5.00
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Kinetochore–microtubule interaction during S phase in Saccharomyces cerevisiae

Etsushi Kitamura, Kozo Tanaka1, Yoko Kitamura, and Tomoyuki U. Tanaka2

Wellcome Trust Centre for Gene Regulation and Expression, College of Life Sciences, University of Dundee, Dundee DD1 5EH, United Kingdom

In the budding yeast Saccharomyces cerevisiae, microtubule-organizing centers called spindle pole bodies (SPBs) are embedded in the nuclear envelope, which remains intact throughout the cell cycle (closed mitosis). Kinetochores are tethered to SPBs by microtubules during most of the cell cycle, including G1 and M phases; however, it has been a topic of debate whether microtubule interaction is constantly maintained or transiently disrupted during chromosome duplication. Here, we show that centromeres are detached from microtubules for 1–2 min and displaced away from a spindle pole in early S phase. These detachment and displacement events are caused by centromere DNA replication, which results in disassembly of kinetochores. Soon afterward, kinetochores are reassembled, leading to their recapture by microtubules. We also show how kinetochores are subsequently transported poleward by microtubules. Our study gives new insights into kinetochore–microtubule interaction and kinetochore duplication during S phase in a closed mitosis.

[Keywords: Kinetochore; microtubule; S phase; Saccharomyces cerevisiae; closed mitosis]]

Received July 17, 2007; revised version accepted October 18, 2007.

1 Present address: Institute of Development, Aging, and Cancer, Tohoku University, Sendai 980-8575, Japan.

2 Corresponding author.

E-MAIL t.tanaka{at}lifesci.dundee.ac.uk; FAX 44-01382-388072.

Supplemental material is available at http://www.genesdev.org.

Article is online at http://www.genesdev.org/cgi/doi/10.1101/gad.449407


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