Genes and Development

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

GENES & DEVELOPMENT 22:1141-1146, 2008
©2008 by Cold Spring Harbor Laboratory Press; ISSN 0890-9369/ $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Research Data
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Google Scholar
Right arrow Articles by Wood, A. J.
Right arrow Articles by Oakey, R. J.
PubMed
Right arrow PubMed Citation
Right arrow Articles by Wood, A. J.
Right arrow Articles by Oakey, R. J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

RESEARCH COMMUNICATION

Regulation of alternative polyadenylation by genomic imprinting

Andrew J. Wood1,4, Reiner Schulz1, Kathryn Woodfine1, Katarzyna Koltowska1, Colin V. Beechey2, Jo Peters2, Deborah Bourc’his3, and Rebecca J. Oakey1,5

1 Department of Medical and Molecular Genetics, King’s College London, Guy’s Hospital, London SE1 9RT, United Kingdom; 2 Medical Research Council Mammalian Genetics Unit, Harwell OX11 0RD, United Kingdom; 3 Institut National de la Santé et de la Recherche Médicale (INSERM) U741, Institut Jacques Monod, 75251 Paris, Cedex 05, France

Maternally and paternally derived alleles can utilize different promoters, but allele-specific differences in cotranscriptional processes have not been reported. We show that alternative polyadenylation sites at a novel murine imprinted gene (H13) are utilized in an allele-specific manner. A differentially methylated CpG island separates polyA sites utilized on maternal and paternal alleles, and contains an internal promoter. Two genetic systems show that alleles lacking methylation generate truncated H13 transcripts that undergo internal polyadenylation. On methylated alleles, the internal promoter is inactive and elongation proceeds to downstream polyadenylation sites. This demonstrates that epigenetic modifications can influence utilization of alternative polyadenylation sites.

[Keywords: Polyadenylation; epigenetics; imprinting; Mcts2]]

Received January 28, 2008; revised version accepted March 10, 2008.

4 Present address: Department of Molecular Cell Biology, University of California at Berkeley, Berkeley, CA 94720, USA.

5 Corresponding author.

E-MAIL rebecca.oakey{at}kcl.ac.uk; FAX 44-207188-2585.

Supplemental material is available at http://www.genesdev.org.

Article is online at http://www.genesdev.org/cgi/doi/10.1101/gad.473408.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?




Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Genome Res. Learn. Mem.
Protein Science RNA Genes Dev.
Copyright © 2008 by Cold Spring Harbor Laboratory Press.