![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Research Papers
Department of Pediatrics, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Abstract
The product of the c-myc proto-oncogene is a DNA-binding protein, the deregulated expression of which is associated with a variety of malignant neoplasms. The cDNA for the max gene was recently cloned as a result of the ability of its protein product to interact with the c-Myc protein. We studied bacterially produced Max, c-Myc, and a series of truncated c-Myc proteins. Full-length c-Myc alone cannot bind DNA. However, a truncated c-Myc protein comprising the basic, helix-loop-helix, and leucine zipper regions can bind specifically to DNA bearing the sequence GGGCAC(G/A)TGCCC. Max protein, either alone or in a heteromeric complex with full-length c-Myc, binds to the same core sequence. Using a novel combination of chemical and photo-cross-linking analysis, we demonstrate that either Max or a c-Myc/Max heteromeric complex binds to DNA virtually exclusively in a dimeric structure. Using fusion proteins in cultured cells, we establish a number of functional characteristics of Max. First, we show that Max can interact with c-Myc intracellularly in a manner dependent on the integrity of the helix-loop-helix and leucine zipper motifs. Second, a nuclear localization domain that contains the sequence PQSRKKLR is mapped to the carboxy-terminal region of Max. Third, Max lacks a transcriptional activation domain that is functional in Chinese hamster ovary cells when fused to a heterologous DNA-binding domain. These data suggest that Max may serve as a cofactor for c-Myc in transcriptional activation or, by itself, as a transcriptional repressor.
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. J. Ravitz, L. Chen, M. Lynch, and E. V. Schmidt c-myc Repression of TSC2 Contributes to Control of Translation Initiation and Myc-Induced Transformation Cancer Res., December 1, 2007; 67(23): 11209 - 11217. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Kawagoe, A. Kandilci, T. A. Kranenburg, and G. C. Grosveld Overexpression of N-Myc Rapidly Causes Acute Myeloid Leukemia in Mice Cancer Res., November 15, 2007; 67(22): 10677 - 10685. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. H. Patel and S. B. McMahon BCL2 Is a Downstream Effector of MIZ-1 Essential for Blocking c-MYC-induced Apoptosis J. Biol. Chem., January 5, 2007; 282(1): 5 - 13. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. W. Hooker and P. J. Hurlin Of Myc and Mnt J. Cell Sci., January 15, 2006; 119(2): 208 - 216. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. Marinkovic, T. Marinkovic, E. Kokai, T. Barth, P. Moller, and T. Wirth Identification of novel Myc target genes with a potential role in lymphomagenesis Nucleic Acids Res., October 11, 2004; 32(18): 5368 - 5378. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. V. Grinberg, C.-D. Hu, and T. K. Kerppola Visualization of Myc/Max/Mad Family Dimers and the Competition for Dimerization in Living Cells Mol. Cell. Biol., May 15, 2004; 24(10): 4294 - 4308. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. Haggerty, K. I. Zeller, R. C. Osthus, D. R. Wonsey, and C. V. Dang A strategy for identifying transcription factor binding sites reveals two classes of genomic c-Myc target sites PNAS, April 29, 2003; 100(9): 5313 - 5318. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Hermann, K. D. Berndt, and A. P. Wright How Transcriptional Activators Bind Target Proteins J. Biol. Chem., October 19, 2001; 276(43): 40127 - 40132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. A. Baudino and J. L. Cleveland The Max Network Gone Mad Mol. Cell. Biol., February 1, 2001; 21(3): 691 - 702. [Full Text]
|
|
|
|
 |
|
 J. L. Hecht and J. C. Aster Molecular Biology of Burkitt's Lymphoma J. Clin. Oncol., November 1, 2000; 18(21): 3707 - 3721. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. J. Wood, M. Mukherjee, C. E. Dolde, Y. Xu, J. F. Maher, T. E. Bunton, J. B. Williams, and L. M. S. Resar HMG-I/Y, a New c-Myc Target Gene and Potential Oncogene Mol. Cell. Biol., August 1, 2000; 20(15): 5490 - 5502. [Abstract] [Full Text]
|
|
|
|
|
|
B. M. Orozco, L.-J. Kong, L. A. Batts, S. Elledge, and L. Hanley-Bowdoin The Multifunctional Character of a Geminivirus Replication Protein Is Reflected by Its Complex Oligomerization Properties J. Biol. Chem., February 25, 2000; 275(9): 6114 - 6122. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. N. Billin, A. L. Eilers, C. Queva, and D. E. Ayer Mlx, a Novel Max-like BHLHZip Protein That Interacts with the Max Network of Transcription Factors J. Biol. Chem., December 17, 1999; 274(51): 36344 - 36350. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. K. Mateyak, A. J. Obaya, and J. M. Sedivy c-Myc Regulates Cyclin D-Cdk4 and -Cdk6 Activity but Affects Cell Cycle Progression at Multiple Independent Points Mol. Cell. Biol., July 1, 1999; 19(7): 4672 - 4683. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Sjöblom-Hallén, W. Yang, A. Jansson, and L. Rymo Silencing of the Epstein-Barr Virus Latent Membrane Protein 1 Gene by the Max-Mad1-mSin3A Modulator of Chromatin Structure J. Virol., April 1, 1999; 73(4): 2983 - 2993. [Abstract] [Full Text]
|
|
|
|
|
|
C. V. Dang c-Myc Target Genes Involved in Cell Growth, Apoptosis, and Metabolism Mol. Cell. Biol., January 1, 1999; 19(1): 1 - 11. [Full Text] [PDF]
|
|
|
|
 |
|
 L. Facchini and L. Z. Penn The molecular role of Myc in growth and transformation: recent discoveries lead to new insights FASEB J, June 1, 1998; 12(9): 633 - 651. [Abstract] [Full Text]
|
|
|
|
|
|
A. Sommer, K. Bousset, E. Kremmer, M. Austen, and B. Luscher Identification and Characterization of Specific DNA-binding Complexes Containing Members of the Myc/Max/Mad Network of Transcriptional Regulators J. Biol. Chem., March 20, 1998; 273(12): 6632 - 6642. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Pinto and C. G. Lobe Products of the grg (Groucho-related Gene) Family Can Dimerize through the Amino-terminal Q Domain J. Biol. Chem., December 20, 1996; 271(51): 33026 - 33031. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T J Hemesath, E Steingrimsson, G McGill, M J Hansen, J Vaught, C A Hodgkinson, H Arnheiter, N G Copeland, N A Jenkins, and D E Fisher microphthalmia, a critical factor in melanocyte development, defines a discrete transcription factor family. Genes & Dev., November 15, 1994; 8(22): 2770 - 2780. [Abstract] [PDF]
|
|
|
|
|
|
S R Hann, M Dixit, R C Sears, and L Sealy The alternatively initiated c-Myc proteins differentially regulate transcription through a noncanonical DNA-binding site. Genes & Dev., October 15, 1994; 8(20): 2441 - 2452. [Abstract] [PDF]
|
|
|
|
|
|
A Giovane, A Pintzas, S M Maira, P Sobieszczuk, and B Wasylyk Net, a new ets transcription factor that is activated by Ras. Genes & Dev., July 1, 1994; 8(13): 1502 - 1513. [Abstract] [PDF]
|
|
|
|
|
|
T Ellenberger, D Fass, M Arnaud, and S C Harrison Crystal structure of transcription factor E47: E-box recognition by a basic region helix-loop-helix dimer. Genes & Dev., April 15, 1994; 8(8): 970 - 980. [Abstract] [PDF]
|
|
|
|
 |
|
 W Gu, K Bhatia, I. Magrath, C. Dang, and R Dalla-Favera Binding and suppression of the Myc transcriptional activation domain by p107 Science, April 8, 1994; 264(5156): 251 - 254. [Abstract] [PDF]
|
|
|
|
|
|
D E Ayer and R N Eisenman A switch from Myc:Max to Mad:Max heterocomplexes accompanies monocyte/macrophage differentiation. Genes & Dev., November 1, 1993; 7(11): 2110 - 2119. [Abstract] [PDF]
|
|
|
|
|
|
K Helin, C L Wu, A R Fattaey, J A Lees, B D Dynlacht, C Ngwu, and E Harlow Heterodimerization of the transcription factors E2F-1 and DP-1 leads to cooperative trans-activation. Genes & Dev., October 1, 1993; 7(10): 1850 - 1861. [Abstract] [PDF]
|
|
|
|
 |
|
 C. Moens, B. Stanton, L. Parada, and J Rossant Defects in heart and lung development in compound heterozygotes for two different targeted mutations at the N-myc locus Development, January 10, 1993; 119(2): 485 - 499. [Abstract] [PDF]
|
|
|
|
|
|
G C Prendergast, R Hopewell, B J Gorham, and E B Ziff Biphasic effect of Max on Myc cotransformation activity and dependence on amino- and carboxy-terminal Max functions. Genes & Dev., December 1, 1992; 6(12a): 2429 - 2439. [Abstract] [PDF]
|
|
|
|
|
|
B Mukherjee, S D Morgenbesser, and R A DePinho Myc family oncoproteins function through a common pathway to transform normal cells in culture: cross-interference by Max and trans-acting dominant mutants. Genes & Dev., August 1, 1992; 6(8): 1480 - 1492. [Abstract] [PDF]
|
|
|
|
|
|
T. P. Makela, P. J. Koskinen, I. Vastrik, and K. Alitalo Alternative Forms of Max as Enhancers or Suppressors of Myc-Ras Cotransformation Science, April 17, 1992; 256(5055): 373 - 377. [Abstract] [PDF]
|
|
