![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Research Papers
Department of Pharmacology, University of Washington School of Medicine, Seattle 98195.
Abstract
This study analyzes the hierarchy of signals that spatially restrict expression of Xenopus Xwnt-8 to mesodermal cells outside of the Spemann organizer field and examines the potential role that endogenous Xwnt-8 may play in dorsoventral patterning of the embryonic mesoderm. The effects of ectopic introduction of a Nieuwkoop center-like activity or of ectopic expression of goosecoid, on the distribution of endogenous Xwnt-8 transcripts were analyzed. The results of these studies are consistent with the hypothesis that maternally derived signals from the Nieuwkoop center function to positively regulate expression of the homeo box gene goosecoid in Spemann organizer cells, leading to a subsequent repression of Xwnt-8 expression in these cells. This exclusion of Xwnt-8 from cells of the organizer field may be important for normal dorsal development, in that ectopic expression of Xwnt-8 in organizer cells after the midblastula stage, by injection of plasmid DNA, ventralizes the fate of these cells. This is distinct from the previously observed dorsalizing effect of Xwnt-8 when expressed prior to the midblastula stage by injection of RNA. The effects of plasmid-derived Xwnt-8 on isolated blastula animal cap ectoderm were also analyzed. Expression of Xwnt-8 in animal pole ectoderm after the midblastula stage ventralizes the response of dorsal animal pole cells to activin and allows naive ectodermal cells to differentiate as ventral mesoderm in the absence of added growth factors. Collectively, these data are consistent with the hypothesis that Xwnt-8 plays a role in the mesodermal differentiation of ventral marginal zone cells during normal development. Furthermore, endogenous Xwnt-8 may ventralize the response of lateral mesodermal cells to dorsalizing signals from the organizer, thus contributing to the graded nature of the final body pattern.
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  H. Zhao, K. Tanegashima, H. Ro, and I. B. Dawid Lrig3 regulates neural crest formation in Xenopus by modulating Fgf and Wnt signaling pathways Development, April 1, 2008; 135(7): 1283 - 1293. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Liu, O. van den Broek, O. Destree, and S. Hoppler Distinct roles for Xenopus Tcf/Lef genes in mediating specific responses to Wnt/{beta}-catenin signalling in mesoderm development Development, December 15, 2005; 132(24): 5375 - 5385. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. E. Reintsch, A. Habring-Mueller, R. W. Wang, A. Schohl, and F. Fagotto {beta}-Catenin controls cell sorting at the notochord-somite boundary independently of cadherin-mediated adhesion J. Cell Biol., August 15, 2005; 170(4): 675 - 686. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Shiotsugu, Y. Katsuyama, K. Arima, A. Baxter, T. Koide, J. Song, R. A. S. Chandraratna, and B. Blumberg Multiple points of interaction between retinoic acid and FGF signaling during embryonic axis formation Development, June 1, 2004; 131(11): 2653 - 2667. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. L. Lewis, J. Bonner, M. Modrell, J. W. Ragland, R. T. Moon, R. I. Dorsky, and D. W. Raible Reiterated Wnt signaling during zebrafish neural crest development Development, March 15, 2004; 131(6): 1299 - 1308. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. L. Blitz, K. W. Y. Cho, and C. Chang Twisted gastrulation loss-of-function analyses support its role as a BMP inhibitor during early Xenopus embryogenesis Development, October 15, 2003; 130(20): 4975 - 4988. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Itasaki, C. M. Jones, S. Mercurio, A. Rowe, P. M. Domingos, J. C. Smith, and R. Krumlauf Wise, a context-dependent activator and inhibitor of Wnt signalling Development, September 15, 2003; 130(18): 4295 - 4305. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Davidson, B. Mao, I. del Barco Barrantes, and C. Niehrs Kremen proteins interact with Dickkopf1 to regulate anteroposterior CNS patterning Development, March 14, 2003; 129(24): 5587 - 5596. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Yang, C. Tan, R. S. Darken, P. A. Wilson, and P. S. Klein {beta}-Catenin/Tcf-regulated transcription prior to the midblastula transition Development, March 14, 2003; 129(24): 5743 - 5752. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. J. Walters, G. A. Wayman, J. C. Notis, R. H. Goodman, T. R. Soderling, and J. L. Christian Calmodulin-dependent protein kinase IV mediated antagonism of BMP signaling regulates lineage and survival of hematopoietic progenitors Development, March 5, 2003; 129(6): 1455 - 1466. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. M. Woda, J. Pastagia, M. Mercola, and K. B. Artinger Dlx proteins position the neural plate border and determine adjacent cell fates Development, March 2, 2003; 130(2): 331 - 342. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 B. K. Brott and S. Y. Sokol Regulation of Wnt/LRP Signaling by Distinct Domains of Dickkopf Proteins Mol. Cell. Biol., September 1, 2002; 22(17): 6100 - 6110. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S.-H. Kim, J. Shin, H.-C. Park, S.-Y. Yeo, S.-K. Hong, S. Han, M. Rhee, C.-H. Kim, A. B. Chitnis, and T.-L. Huh Specification of an anterior neuroectoderm patterning by Frizzled8a-mediated Wnt8b signalling during late gastrulation in zebrafish Development, January 10, 2002; 129(19): 4443 - 4455. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Schohl and F. Fagotto {beta}-catenin, MAPK and Smad signaling during early Xenopus development Development, January 1, 2002; 129(1): 37 - 52. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. Kiecker and C. Niehrs A morphogen gradient of Wnt/{beta}-catenin signalling regulates anteroposterior neural patterning in Xenopus Development, November 1, 2001; 128(21): 4189 - 4201. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. van de Water, M. van de Wetering, J. Joore, J. Esseling, R. Bink, H. Clevers, and D. Zivkovic Ectopic Wnt signal determines the eyeless phenotype of zebrafish masterblind mutant Development, October 15, 2001; 128(20): 3877 - 3888. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Yasuo and P. Lemaire Role of Goosecoid, Xnot and Wnt antagonists in the maintenance of the notochord genetic programme in Xenopus gastrulae Development, October 1, 2001; 128(19): 3783 - 3793. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. S. Yamamoto, C. Takagi, A. C. Hyodo, and N. Ueno Suppression of head formation by Xmsx-1 through the inhibition of intracellular nodal signaling Development, July 15, 2001; 128(14): 2769 - 2779. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. S. Hamilton, G. N. Wheeler, and S. Hoppler Difference in XTcf-3 dependency accounts for change in response to {beta}-catenin-mediated Wnt signalling in Xenopus blastula Development, June 1, 2001; 128(11): 2063 - 2073. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. A. Schneider and M. Mercola Wnt antagonism initiates cardiogenesis in Xenopus laevis Genes & Dev., February 1, 2001; 15(3): 304 - 315. [Abstract] [Full Text]
|
|
|
|
|
|
M. J. Marvin, G. Di Rocco, A. Gardiner, S. M. Bush, and A. B. Lassar Inhibition of Wnt activity induces heart formation from posterior mesoderm Genes & Dev., February 1, 2001; 15(3): 316 - 327. [Abstract] [Full Text]
|
|
|
|
|
|
H. Standley, A. Zorn, and J. Gurdon eFGF and its mode of action in the community effect during Xenopus myogenesis Development, January 4, 2001; 128(8): 1347 - 1357. [Abstract] [PDF]
|
|
|
|
|
|
G. A. Wayman, M. J. Walters, K. Kolibaba, T. R. Soderling, and J. L. Christian CaM Kinase IV Regulates Lineage Commitment and Survival of Erythroid Progenitors in a Non-Cell-Autonomous Manner J. Cell Biol., November 13, 2000; 151(4): 811 - 824. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W. Montross, H Ji, and P. McCrea A beta-catenin/engrailed chimera selectively suppresses Wnt signaling J. Cell Sci., May 1, 2000; 113(10): 1759 - 1770. [Abstract] [PDF]
|
|
|
|
 |
|
 D. B. Sparrow, B. Latinkic, and T. J. Mohun A simplified method of generating transgenic Xenopus Nucleic Acids Res., February 15, 2000; 28(4): e12 - e12. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O Kazanskaya, A Glinka, and C Niehrs The role of Xenopus dickkopf1 in prechordal plate specification and neural patterning Development, January 11, 2000; 127(22): 4981 - 4992. [Abstract] [PDF]
|
|
|
|
|
|
C Wu, Q Zeng, K. Blumer, and A. Muslin RGS proteins inhibit Xwnt-8 signaling in Xenopus embryonic development Development, January 7, 2000; 127(13): 2773 - 2784. [Abstract] [PDF]
|
|
|
|
|
|
S Sumanas, P Strege, J Heasman, and S. Ekker The putative wnt receptor Xenopus frizzled-7 functions upstream of beta-catenin in vertebrate dorsoventral mesoderm patterning Development, January 5, 2000; 127(9): 1981 - 1990. [Abstract] [PDF]
|
|
|
|
|
|
J. C. Baker, R. S.P. Beddington, and R. M. Harland Wnt signaling in Xenopus embryos inhibits Bmp4 expression and activates neural development Genes & Dev., December 1, 1999; 13(23): 3149 - 3159. [Abstract] [Full Text]
|
|
|
|
 |
|
 Y. Kato, Y. Shi, and X. He Neuralization of the Xenopus Embryo by Inhibition of p300/ CREB-Binding Protein Function J. Neurosci., November 1, 1999; 19(21): 9364 - 9373. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Itoh and S. Y. Sokol Axis determination by inhibition of Wnt signaling in Xenopus Genes & Dev., September 1, 1999; 13(17): 2328 - 2336. [Abstract] [Full Text]
|
|
|
|
|
|
G Kumano, L Belluzzi, and W. Smith Spatial and temporal properties of ventral blood island induction in Xenopus laevis Development, January 12, 1999; 126(23): 5327 - 5337. [Abstract] [PDF]
|
|
|
|
|
|
K Wunnenberg-Stapleton, I. Blitz, C Hashimoto, and K. Cho Involvement of the small GTPases XRhoA and XRnd1 in cell adhesion and head formation in early Xenopus development Development, January 12, 1999; 126(23): 5339 - 5351. [Abstract] [PDF]
|
|
|
|
|
|
U Borello, M Coletta, S Tajbakhsh, L Leyns, E. De Robertis, M Buckingham, and G Cossu Transplacental delivery of the Wnt antagonist Frzb1 inhibits development of caudal paraxial mesoderm and skeletal myogenesis in mouse embryos Development, January 10, 1999; 126(19): 4247 - 4255. [Abstract] [PDF]
|
|
|
|
|
|
Q Tian, T Nakayama, M. Dixon, and J. Christian Post-transcriptional regulation of Xwnt-8 expression is required for normal myogenesis during vertebrate embryonic development Development, January 8, 1999; 126(15): 3371 - 3380. [Abstract] [PDF]
|
|
|
|
 |
|
 D. J. Van Den Berg, A. K. Sharma, E. Bruno, and R. Hoffman Role of Members of the Wnt Gene Family in Human Hematopoiesis Blood, November 1, 1998; 92(9): 3189 - 3202. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Behrens, B. Jerchow, M. Würtele, J. Grimm, C. Asbrand, R. Wirtz, M. Kühl, D. Wedlich, and W. Birchmeier Functional Interaction of an Axin Homolog, Conductin, with  -Catenin, APC, and GSK3Science, April 24, 1998; 280(5363): 596 - 599. [Abstract] [Full Text]
|
|
|
|
|
|
T. Sykes, A. Rodaway, M. Walmsley, and R. Patient Suppression of GATA factor activity causes axis duplication in Xenopus Development, January 12, 1998; 125(23): 4595 - 4605. [Abstract] [PDF]
|
|
|
|
|
|
Q Xu, P. D'Amore, and S. Sokol Functional and biochemical interactions of Wnts with FrzA, a secreted Wnt antagonist Development, January 12, 1998; 125(23): 4767 - 4776. [Abstract] [PDF]
|
|
|
|
|
|
L. Wu and J. Lengyel Role of caudal in hindgut specification and gastrulation suggests homology between Drosophila amnioproctodeal invagination and vertebrate blastopore Development, January 7, 1998; 125(13): 2433 - 2442. [Abstract] [PDF]
|
|
|
|
|
|
F Emily-Fenouil, C Ghiglione, G Lhomond, T Lepage, and C Gache GSK3beta/shaggy mediates patterning along the animal-vegetal axis of the sea urchin embryo Development, January 7, 1998; 125(13): 2489 - 2498. [Abstract] [PDF]
|
|
|
|
|
|
M. Deardorff, C Tan, L. Conrad, and P. Klein Frizzled-8 is expressed in the Spemann organizer and plays a role in early morphogenesis Development, January 7, 1998; 125(14): 2687 - 2700. [Abstract] [PDF]
|
|
|
|
|
|
S. Amacher and C. Kimmel Promoting notochord fate and repressing muscle development in zebrafish axial mesoderm Development, January 4, 1998; 125(8): 1397 - 1406. [Abstract] [PDF]
|
|
|
|
|
|
D Onichtchouk, A Glinka, and C Niehrs Requirement for Xvent-1 and Xvent-2 gene function in dorsoventral patterning of Xenopus mesoderm Development, January 4, 1998; 125(8): 1447 - 1456. [Abstract] [PDF]
|
|
|
|
|
|
T Nakayama, M. Snyder, S. Grewal, K Tsuneizumi, T Tabata, and J. Christian Xenopus Smad8 acts downstream of BMP-4 to modulate its activity during vertebrate embryonic patterning Development, January 3, 1998; 125(5): 857 - 867. [Abstract] [PDF]
|
|
|
|
|
|
K. M. Cadigan and R. Nusse Wnt signaling: a common theme in animal development Genes & Dev., December 15, 1997; 11(24): 3286 - 3305. [Full Text] [PDF]
|
|
|
|
 |
|
 J.-P. Saint-Jeannet, X. He, H. E. Varmus, and I. B. Dawid Regulation of dorsal fate in the neuraxis by Wnt-1 and Wnt-3a PNAS, December 9, 1997; 94(25): 13713 - 13718. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. W. Austin, G. P. Solar, F. C. Ziegler, L. Liem, and W. Matthews A Role for the Wnt Gene Family in Hematopoiesis: Expansion of Multilineage Progenitor Cells Blood, May 15, 1997; 89(10): 3624 - 3635. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. He, J.-P. Saint-Jeannet, Y. Wang, J. Nathans, I. Dawid, and H. Varmus A Member of the Frizzled Protein Family Mediating Axis Induction by Wnt-5A Science, March 14, 1997; 275(5306): 1652 - 1654. [Abstract] [Full Text]
|
|
|
|
|
|
M. Laurent, I. Blitz, C Hashimoto, U Rothbacher, and K. Cho The Xenopus homeobox gene twin mediates Wnt induction of goosecoid in establishment of Spemann's organizer Development, January 12, 1997; 124(23): 4905 - 4916. [Abstract] [PDF]
|
|
|
|
|
|
M Furthauer, C Thisse, and B Thisse A role for FGF-8 in the dorsoventral patterning of the zebrafish gastrula Development, January 11, 1997; 124(21): 4253 - 4264. [Abstract] [PDF]
|
|
|
|
|
|
H Popperl, C Schmidt, V Wilson, C. Hume, J Dodd, R Krumlauf, and R. Beddington Misexpression of Cwnt8C in the mouse induces an ectopic embryonic axis and causes a truncation of the anterior neuroectoderm Development, January 8, 1997; 124(15): 2997 - 3005. [Abstract] [PDF]
|
|
|
|
|
|
R Maeda, A Kobayashi, R Sekine, J. Lin, H Kung, and M Maeno Xmsx-1 modifies mesodermal tissue pattern along dorsoventral axis in Xenopus laevis embryo Development, January 7, 1997; 124(13): 2553 - 2560. [Abstract] [PDF]
|
|
|
|
|
|
M Tada, M. O'Reilly, and J. Smith Analysis of competence and of Brachyury autoinduction by use of hormone-inducible Xbra Development, January 6, 1997; 124(11): 2225 - 2234. [Abstract] [PDF]
|
|
|
|
|
|
R Dosch, V Gawantka, H Delius, C Blumenstock, and C Niehrs Bmp-4 acts as a morphogen in dorsoventral mesoderm patterning in Xenopus Development, January 6, 1997; 124(12): 2325 - 2334. [Abstract] [PDF]
|
|
|
|
|
|
S Fisher, S. Amacher, and M. Halpern Loss of cerebum function ventralizes the zebrafish embryo Development, January 4, 1997; 124(7): 1301 - 1311. [Abstract] [PDF]
|
|
|
|
|
|
M. Mullins, M Hammerschmidt, D. Kane, J Odenthal, M Brand, F. van Eeden, M Furutani-Seiki, M Granato, P Haffter, C. Heisenberg, et al. Genes establishing dorsoventral pattern formation in the zebrafish embryo: the ventral specifying genes Development, January 12, 1996; 123(1): 81 - 93. [Abstract] [PDF]
|
|
|
|
|
|
J Zhang and M. King Xenopus VegT RNA is localized to the vegetal cortex during oogenesis and encodes a novel T-box transcription factor involved in mesodermal patterning Development, January 12, 1996; 122(12): 4119 - 4129. [Abstract] [PDF]
|
|
|
|
|
|
D Onichtchouk, V Gawantka, R Dosch, H Delius, K Hirschfeld, C Blumenstock, and C Niehrs The Xvent-2 homeobox gene is part of the BMP-4 signalling pathway controlling [correction of controling] dorsoventral patterning of Xenopus mesoderm Development, January 10, 1996; 122(10): 3045 - 3053. [Abstract] [PDF]
|
|
|
|
|
