![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Research Papers
Laboratory of Molecular Embryology, National Institute of Child Health and Human Development, Bethesda, Maryland 20892.
Abstract
The chromatin assembly process coupled to DNA synthesis in the Xenopus oocyte nucleus is significantly more repressive toward basal transcription than chromatin assembly on duplex DNA. We show that chromatin assembly concurrent with DNA synthesis over the promoter region itself is causal for repression. However, the trans-activator Gal4-VP16 both relieves repression and activates transcription regardless of the chromatin assembly pathway. This activation is independent of whether Gal4-VP16 addition occurs before or after chromatin assembly. We propose that replication-coupled chromatin assembly represents a general mechanism to direct the efficient repression of basal transcription. However transcription induction by a specific activator, Gal4-VP16, occurs independent of this chromatin-mediated repression.
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J. M. Hernandez, A. Feller, K. Morohashi, K. Frame, and E. Grotewold The basic helix loop helix domain of maize R links transcriptional regulation and histone modifications by recruitment of an EMSY-related factor PNAS, October 23, 2007; 104(43): 17222 - 17227. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Belikov, C. Astrand, and O. Wrange Mechanism of Histone H1-Stimulated Glucocorticoid Receptor DNA Binding In Vivo Mol. Cell. Biol., March 15, 2007; 27(6): 2398 - 2410. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Li, J. Fu, C. Toumazou, H.-G. Yoon, and J. Wong A Role of the Amino-Terminal (N) and Carboxyl-Terminal (C) Interaction in Binding of Androgen Receptor to Chromatin Mol. Endocrinol., April 1, 2006; 20(4): 776 - 785. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C. J. Burd, C. E. Petre, L. M. Morey, Y. Wang, M. P. Revelo, C. A. Haiman, S. Lu, C. M. Fenoglio-Preiser, J. Li, E. S. Knudsen, et al. Cyclin D1b variant influences prostate cancer growth through aberrant androgen receptor regulation PNAS, February 14, 2006; 103(7): 2190 - 2195. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Stewart, J. Li, and J. Wong Relationship between Histone H3 Lysine 9 Methylation, Transcription Repression, and Heterochromatin Protein 1 Recruitment Mol. Cell. Biol., April 1, 2005; 25(7): 2525 - 2538. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Kievit and R. A. Maurer The Pituitary-Specific Transcription Factor, Pit-1, Can Direct Changes in the Chromatin Structure of the Prolactin Promoter Mol. Endocrinol., January 1, 2005; 19(1): 138 - 147. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Belikov, P.-H. Holmqvist, C. Astrand, and O. Wrange Nuclear Factor 1 and Octamer Transcription Factor 1 Binding Preset the Chromatin Structure of the Mouse Mammary Tumor Virus Promoter for Hormone Induction J. Biol. Chem., November 26, 2004; 279(48): 49857 - 49867. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Belikov, C. Astrand, P.-H. Holmqvist, and O. Wrange Chromatin-Mediated Restriction of Nuclear Factor 1/CTF Binding in a Repressed and Hormone-Activated Promoter In Vivo Mol. Cell. Biol., April 1, 2004; 24(7): 3036 - 3047. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Barbie, B. A. Kudlow, R. Frock, J. Zhao, B. R. Johnson, N. Dyson, E. Harlow, and B. K. Kennedy Nuclear Reorganization of Mammalian DNA Synthesis Prior to Cell Cycle Exit Mol. Cell. Biol., January 15, 2004; 24(2): 595 - 607. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. Bjork, G. Bauren, B. Gelius, O. Wrange, and L. Wieslander The Chironomus tentans translation initiation factor eIF4H is present in the nucleus but does not bind to mRNA until the mRNA reaches the cytoplasmic perinuclear region J. Cell Sci., November 15, 2003; 116(22): 4521 - 4532. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. M. Sachs, P. L. Jones, E. Havis, N. Rouse, B. A. Demeneix, and Y.-B. Shi Nuclear Receptor Corepressor Recruitment by Unliganded Thyroid Hormone Receptor in Gene Repression during Xenopus laevis Development Mol. Cell. Biol., December 15, 2002; 22(24): 8527 - 8538. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. A. Irvine, I. G. Lin, and C.-L. Hsieh DNA Methylation Has a Local Effect on Transcription and Histone Acetylation Mol. Cell. Biol., October 1, 2002; 22(19): 6689 - 6696. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Li, Q. Lin, H.-G. Yoon, Z.-Q. Huang, B. D. Strahl, C. D. Allis, and J. Wong Involvement of Histone Methylation and Phosphorylation in Regulation of Transcription by Thyroid Hormone Receptor Mol. Cell. Biol., August 15, 2002; 22(16): 5688 - 5697. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Curradi, A. Izzo, G. Badaracco, and N. Landsberger Molecular Mechanisms of Gene Silencing Mediated by DNA Methylation Mol. Cell. Biol., May 1, 2002; 22(9): 3157 - 3173. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Z.-Q. Huang, J. Li, and J. Wong AR Possesses an Intrinsic Hormone-Independent Transcriptional Activity Mol. Endocrinol., May 1, 2002; 16(5): 924 - 937. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. Li, Q. Lin, W. Wang, P. Wade, and J. Wong Specific targeting and constitutive association of histone deacetylase complexes during transcriptional repression Genes & Dev., March 15, 2002; 16(6): 687 - 692. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Rastelli, K. Robinson, Y. Xu, and S. Majumder Reconstitution of Enhancer Function in Paternal Pronuclei of One-Cell Mouse Embryos Mol. Cell. Biol., August 15, 2001; 21(16): 5531 - 5540. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Matsumoto, K. Aoki, N. Dohmae, K. Takio, and M. Tsujimoto CIRP2, a major cytoplasmic RNA-binding protein in Xenopus oocytes Nucleic Acids Res., December 1, 2000; 28(23): 4689 - 4697. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. Albagli, C. Lindon, D. Lantoine, S. Quief, E. Puvion, C. Pinset, and F. Puvion-Dutilleul DNA Replication Progresses on the Periphery of Nuclear Aggregates Formed by the BCL6 Transcription Factor Mol. Cell. Biol., November 15, 2000; 20(22): 8560 - 8570. [Abstract] [Full Text]
|
|
|
|
|
|
T. M. Yusufzai and A. P. Wolffe Functional consequences of Rett syndrome mutations on human MeCP2 Nucleic Acids Res., November 1, 2000; 28(21): 4172 - 4179. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Mao and D. J. Shapiro A Histone Deacetylase Inhibitor Potentiates Estrogen Receptor Activation of a Stably Integrated Vitellogenin Promoter in HepG2 Cells Endocrinology, July 1, 2000; 141(7): 2361 - 2369. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. K. Kaludov and A. P. Wolffe MeCP2 driven transcriptional repression in vitro: selectivity for methylated DNA, action at a distance and contacts with the basal transcription machinery Nucleic Acids Res., May 1, 2000; 28(9): 1921 - 1928. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Li, B. W. O'Malley, and J. Wong p300 Requires Its Histone Acetyltransferase Activity and SRC-1 Interaction Domain To Facilitate Thyroid Hormone Receptor Activation in Chromatin Mol. Cell. Biol., March 15, 2000; 20(6): 2031 - 2042. [Abstract] [Full Text]
|
|
|
|
|
|
J. G. Moggs, P. Grandi, J.-P. Quivy, Z. O. Jónsson, U. Hübscher, P. B. Becker, and G. Almouzni A CAF-1-PCNA-Mediated Chromatin Assembly Pathway Triggered by Sensing DNA Damage Mol. Cell. Biol., February 15, 2000; 20(4): 1206 - 1218. [Abstract] [Full Text]
|
|
|
|
|
|
P Ridgway and G Almouzni CAF-1 and the inheritance of chromatin states: at the crossroads of DNA replication and repair J. Cell Sci., January 8, 2000; 113(15): 2647 - 2658. [Abstract] [PDF]
|
|
|
|
|
|
N Kikyo and A. Wolffe Reprogramming nuclei: insights from cloning, nuclear transfer and heterokaryons J. Cell Sci., January 1, 2000; 113(1): 11 - 20. [Abstract] [PDF]
|
|
|
|
|
|
L. B. Smilenov, S. Dhar, and T. K. Pandita Altered Telomere Nuclear Matrix Interactions and Nucleosomal Periodicity in Ataxia Telangiectasia Cells before and after Ionizing Radiation Treatment Mol. Cell. Biol., October 1, 1999; 19(10): 6963 - 6971. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Vermaak, P. A. Wade, P. L. Jones, Y.-B. Shi, and A. P. Wolffe Functional Analysis of the SIN3-Histone Deacetylase RPD3-RbAp48-Histone H4 Connection in the Xenopus Oocyte Mol. Cell. Biol., September 1, 1999; 19(9): 5847 - 5860. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. I. Yarden and L. C. Brody BRCA1 interacts with components of the histone deacetylase complex PNAS, April 27, 1999; 96(9): 4983 - 4988. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
P. Lawinger, L. Rastelli, Z. Zhao, and S. Majumder Lack of Enhancer Function in Mammals Is Unique to Oocytes and Fertilized Eggs J. Biol. Chem., March 19, 1999; 274(12): 8002 - 8011. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. S. Geraghty, H. B. Sucic, J. Chen, and D. S. Pederson Evidence That Partial Unwrapping of DNA from Nucleosomes Facilitates the Binding of Heat Shock Factor following DNA Replication in Yeast J. Biol. Chem., August 7, 1998; 273(32): 20463 - 20472. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Wong, V. C.-T. Liang, L. M. Sachs, and Y.-B. Shi Transcription from the Thyroid Hormone-dependent Promoter of the Xenopus laevis Thyroid Hormone Receptor beta A Gene Requires a Novel Upstream Element and the Initiator, but Not a TATA Box J. Biol. Chem., June 5, 1998; 273(23): 14186 - 14193. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. Minucci, J. Wong, J. C. G. Blanco, Y.-B. Shi, A. P. Wolffe, and K. Ozato Retinoid Receptor-Induced Alteration of the Chromatin Assembled on a Ligand-Responsive Promoter in Xenopus Oocytes Mol. Endocrinol., March 1, 1998; 12(3): 315 - 324. [Abstract] [Full Text]
|
|
|
|
|
|
F. Meric, K. Matsumoto, and A. P. Wolffe Regulated Unmasking of in Vivo Synthesized Maternal mRNA at Oocyte Maturation. A ROLE FOR THE CHAPERONE NUCLEOPLASMIN J. Biol. Chem., May 9, 1997; 272(19): 12840 - 12846. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Puzianowska-Kuznicka, J. Wong, A. Kanamori, and Y.-B. Shi Functional Characterization of a Mutant Thyroid Hormone Receptor in Xenopus laevis J. Biol. Chem., December 27, 1996; 271(52): 33394 - 33403. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Meric, A. M. Searfoss, M. Wormington, and A. P. Wolffe Masking and Unmasking Maternal mRNA. THE ROLE OF POLYADENYLATION, TRANSCRIPTION, SPLICING, AND NUCLEAR HISTORY J. Biol. Chem., November 29, 1996; 271(48): 30804 - 30810. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Matsumoto, F. Meric, and A. P. Wolffe Translational Repression Dependent on the Interaction of the Xenopus Y-box Protein FRGY2 with mRNA. ROLE OF THE COLD SHOCK DOMAIN, TAIL DOMAIN, AND SELECTIVE RNA SEQUENCE RECOGNITION J. Biol. Chem., September 13, 1996; 271(37): 22706 - 22712. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J Wong, Y B Shi, and A P Wolffe A role for nucleosome assembly in both silencing and activation of the Xenopus TR beta A gene by the thyroid hormone receptor. Genes & Dev., November 1, 1995; 9(21): 2696 - 2711. [Abstract] [PDF]
|
|
|
|
|
|
M C Barton and B M Emerson Regulated expression of the beta-globin gene locus in synthetic nuclei. Genes & Dev., October 15, 1994; 8(20): 2453 - 2465. [Abstract] [PDF]
|
|
|
|
 |
|
 C Klein and K Struhl Increased recruitment of TATA-binding protein to the promoter by transcriptional activation domains in vivo Science, October 14, 1994; 266(5183): 280 - 282. [Abstract] [PDF]
|
|
|
|
|
|
D. Robyr, A. Gegonne, A. P. Wolffe, and W. Wahli Determinants of Vitellogenin B1 Promoter Architecture. HNF3 AND ESTROGEN RESPONSIVE TRANSCRIPTION WITHIN CHROMATIN J. Biol. Chem., September 1, 2000; 275(36): 28291 - 28300. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. D. Urnov and A. P. Wolffe An Array of Positioned Nucleosomes Potentiates Thyroid Hormone Receptor Action in Vivo J. Biol. Chem., June 1, 2001; 276(23): 19753 - 19761. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. N. Collingwood, F. D. Urnov, V. K. K. Chatterjee, and A. P. Wolffe Chromatin Remodeling by the Thyroid Hormone Receptor in Regulation of the Thyroid-stimulating Hormone alpha -Subunit Promoter J. Biol. Chem., August 31, 2001; 276(36): 34227 - 34234. [Abstract] [Full Text] [PDF]
|
|
