![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
![[Contents]](/icons/banner/tocACT.gif){kind=icon}
|
|
|
|
|
||
Research Papers
Department of Microbiology and Immunology, Vanderbilt University School of Medicine, Nashville, Tennessee 37232-2363.
Abstract
A gene has been characterized that is required for postimplantation mouse development. The gene, designated fug1, was disrupted in embryonic stem cells by the U3Neo gene trap retrovirus, and the disrupted allele was introduced into the germ line. Homozygous mutant embryos arrest at the egg cylinder stage at about embryonic day 6 and are mostly resorbed by day 8.5. The appearance of the proamniotic cavity is delayed, and epiblast cells that surround the cavity are disorganized. fug1 transcripts are undetectable at E6 but are induced throughout the embryo after E6.5. The gene is expressed at low levels in all adult tissues examined, maps to chromosome 15, and is conserved among mammals. The cDNA sequence encodes a protein of 589 amino acids, the first 400 of which are 38% identical to the Saccaromyces cerevisiae RNA1 gene. Regions of greatest similarity include a long acidic domain and 11 leucine-rich motifs, thought to mediate high affinity protein-protein interactions. These similarities suggest that Fug1 may be required for developmental changes in RNA processing or chromatin structure prior to gastrulation.
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  J.L. Stanton and D.P.L. Green Meta-analysis of gene expression in mouse preimplantation embryo development Mol. Hum. Reprod., June 1, 2001; 7(6): 545 - 552. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. R. Pawlak, C. A. Scherer, J. Chen, M. J. Roshon, and H. E. Ruley Arginine N-Methyltransferase 1 Is Required for Early Postimplantation Mouse Development, but Cells Deficient in the Enzyme Are Viable Mol. Cell. Biol., July 1, 2000; 20(13): 4859 - 4869. [Abstract] [Full Text]
|
|
|
|
|
|
D. J. Williamson, S. Banik-Maiti, J. DeGregori, and H. E. Ruley hnRNP C Is Required for Postimplantation Mouse Development but Is Dispensable for Cell Viability Mol. Cell. Biol., June 1, 2000; 20(11): 4094 - 4105. [Abstract] [Full Text]
|
|
|
|
 |
|
 W. T. Pu, K. Wickman, and D. E. Clapham ICln Is Essential for Cellular and Early Embryonic Viability J. Biol. Chem., April 21, 2000; 275(17): 12363 - 12366. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. E. Donohoe, X. Zhang, L. McGinnis, J. Biggers, E. Li, and Y. Shi Targeted Disruption of Mouse Yin Yang 1 Transcription Factor Results in Peri-Implantation Lethality Mol. Cell. Biol., October 1, 1999; 19(10): 7237 - 7244. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 W Feng, A. Benko, J. Lee, D. Stanford, and A. Hopper Antagonistic effects of NES and NLS motifs determine S. cerevisiae Rna1p subcellular distribution J. Cell Sci., January 2, 1999; 112(3): 339 - 347. [Abstract] [PDF]
|
|
|
|
 |
|
 W. L. Stanford, G. Caruana, K. A. Vallis, M. Inamdar, M. Hidaka, V. L. Bautch, and A. Bernstein Expression Trapping: Identification of Novel Genes Expressed in Hematopoietic and Endothelial Lineages by Gene Trapping in ES Cells Blood, December 15, 1998; 92(12): 4622 - 4631. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Andreu, T. Beckers, E. Thoenes, P. Hilgard, and H. von Melchner Gene Trapping Identifies Inhibitors of Oncogenic Transformation. THE TISSUE INHIBITOR OF METALLOPROTEINASES-3 (TIMP3) AND COLLAGEN TYPE I alpha 2 (COL1A2) ARE EPIDERMAL GROWTH FACTOR-REGULATED GROWTH REPRESSORS J. Biol. Chem., May 29, 1998; 273(22): 13848 - 13854. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
I. S. Thorey, K. Muth, A. P. Russ, J. Otte, A. Reffelmann, and H. von Melchner Selective Disruption of Genes Transiently Induced in Differentiating Mouse Embryonic Stem Cells by Using Gene Trap Mutagenesis and Site-Specific Recombination Mol. Cell. Biol., May 1, 1998; 18(5): 3081 - 3088. [Abstract] [Full Text]
|
|
|
|
 |
|
 R. Mahajan, L. Gerace, and F. Melchior Molecular Characterization of the SUMO-1 Modification of RanGAP1 and Its Role in Nuclear Envelope Association J. Cell Biol., January 26, 1998; 140(2): 259 - 270. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
J. Haberland, J. Becker, and V. Gerke The Acidic C-terminal Domain of rna1p Is Required for the Binding of Ran·GTP and for RanGAP Activity J. Biol. Chem., September 26, 1997; 272(39): 24717 - 24726. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Kamitani, H. P. Nguyen, and E. T. H. Yeh Preferential Modification of Nuclear Proteins by a Novel Ubiquitin-like Molecule J. Biol. Chem., May 30, 1997; 272(22): 14001 - 14004. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Saitoh, R. Pu, M. Cavenagh, and M. Dasso RanBP2 associates with Ubc9p and a modified form of RanGAP1 PNAS, April 15, 1997; 94(8): 3736 - 3741. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S C Farmer, C W Sun, G E Winnier, B L Hogan, and T M Townes The bZIP transcription factor LCR-F1 is essential for mesoderm formation in mouse development. Genes & Dev., March 15, 1997; 11(6): 786 - 798. [Abstract] [PDF]
|
|
|
|
 |
|
 D J Townley, B J Avery, B Rosen, and W C Skarnes Rapid sequence analysis of gene trap integrations to generate a resource of insertional mutations in mice. Genome Res., March 1, 1997; 7(3): 293 - 298. [Abstract] [PDF]
|
|
|
|
|
|
A Battistoni, G Guarguaglini, F Degrassi, C Pittoggi, A Palena, G Di Matteo, C Pisano, E Cundari, and P Lavia Deregulated expression of the RanBP1 gene alters cell cycle progression in murine fibroblasts J. Cell Sci., January 10, 1997; 110(19): 2345 - 2357. [Abstract] [PDF]
|
|
|
|
|
|
K. M. Lounsbury and I. G. Macara Ran-binding Protein 1(RanBP1) Forms a Ternary Complex with Ran and Karyopherin beta and Reduces Ran GTPase-activating Protein (RanGAP) Inhibition by Karyopherin beta J. Biol. Chem., January 3, 1997; 272(1): 551 - 555. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. P. Russ, C. Friedel, K. Ballas, U. Kalina, D. Zahn, K. Strebhardt, and H. von Melchner Identification of genes induced by factor deprivation in hematopoietic cells undergoing apoptosis using gene-trap mutagenesis and site-specific recombination PNAS, December 24, 1996; 93(26): 15279 - 15284. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. M. Lounsbury, S. A. Richards, K. L. Carey, and I. G. Macara Mutations within the Ran/TC4 GTPase. EFFECTS ON REGULATORY FACTOR INTERACTIONS AND SUBCELLULAR LOCALIZATION J. Biol. Chem., December 20, 1996; 271(51): 32834 - 32841. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
C Y Liu, A Flesken-Nikitin, S Li, Y Zeng, and W H Lee Inactivation of the mouse Brca1 gene leads to failure in the morphogenesis of the egg cylinder in early postimplantation development. Genes & Dev., July 15, 1996; 10(14): 1835 - 1843. [Abstract] [PDF]
|
|
|
|
|
|
T Takeuchi, Y Yamazaki, Y Katoh-Fukui, R Tsuchiya, S Kondo, J Motoyama, and T Higashinakagawa Gene trap capture of a novel mouse gene, jumonji, required for neural tube formation. Genes & Dev., May 15, 1995; 9(10): 1211 - 1222. [Abstract] [PDF]
|
|
|
|
 |
|
 J. Cross, Z Werb, and S. Fisher Implantation and the placenta: key pieces of the development puzzle Science, December 2, 1994; 266(5190): 1508 - 1518. [Abstract] [PDF]
|
|
|
|
|
|
W S Chen, K Manova, D C Weinstein, S A Duncan, A S Plump, V R Prezioso, R F Bachvarova, and J E Darnell Disruption of the HNF-4 gene, expressed in visceral endoderm, leads to cell death in embryonic ectoderm and impaired gastrulation of mouse embryos. Genes & Dev., October 15, 1994; 8(20): 2466 - 2477. [Abstract] [PDF]
|
|
|
|
|
|
D D Spyropoulos and M R Capecchi Targeted disruption of the even-skipped gene, evx1, causes early postimplantation lethality of the mouse conceptus. Genes & Dev., August 15, 1994; 8(16): 1949 - 1961. [Abstract] [PDF]
|
|
