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Research Papers
Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
Abstract
The conserved regulators of cell cycle progression--Cyclins, Cdc2 kinase, and String phosphatase (Cdc25)--accommodate multiple modes of regulation during Drosophila embryogenesis. During cell cycles 2-7, Cdc2/Cyclin complexes are continuously present and show little fluctuation in abundance, phosphomodification, or activity. This suggests that cycling of the mitotic apparatus does not require cytoplasmic oscillations of known regulatory activities. During cycles 8-13 a progressive increase in the degradation of Cyclins at mitosis leads to increasing oscillations of Cdc2 kinase activity. Mutants deficient in cyclin mRNAs suffer cell cycle delays during this period, suggesting that Cyclin accumulation times these cycles. During interphase 14, programmed degradation of maternal String protein leads to inhibitory phosphorylation of Cdc2 and cell cycle arrest. Subsequently, mitoses 14-16 are triggered by pulses of zygotic string transcription.
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|
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|
|
|
|
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|
|
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|
|
|
|
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|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
 |
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
|
|
D. Lehman, B Patterson, L. Johnston, T Balzer, J. Britton, R Saint, and B. Edgar Cis-regulatory elements of the mitotic regulator, string/Cdc25 Development, January 5, 1999; 126(9): 1793 - 1803. [Abstract] [PDF]
|
|
|
|
|
|
T. T.-L. Tang, S. E. Bickel, L. M. Young, and T. L. Orr-Weaver Maintenance of sister-chromatid cohesion at the centromere by the Drosophila MEI-S332 protein Genes & Dev., December 15, 1998; 12(24): 3843 - 3856. [Abstract] [Full Text]
|
|
|
|
|
|
D. Stuart and C. Wittenberg CLB5 and CLB6 are required for premeiotic DNA replication and activation of the meiotic S/M checkpoint Genes & Dev., September 1, 1998; 12(17): 2698 - 2710. [Abstract] [Full Text]
|
|
|
|
|
|
T. T. Su, F. Sprenger, P. J. DiGregorio, S. D. Campbell, and P. H. O'Farrell Exit from mitosis in Drosophila syncytial embryos requires proteolysis and cyclin degradation, and is associated with localized dephosphorylation Genes & Dev., May 15, 1998; 12(10): 1495 - 1503. [Abstract] [Full Text]
|
|
|
|
|
|
S. Larochelle, J. Pandur, R. P. Fisher, H. K. Salz, and B. Suter Cdk7 is essential for mitosis and for in vivo Cdk-activating kinase activity Genes & Dev., February 1, 1998; 12(3): 370 - 381. [Abstract] [Full Text]
|
|
|
|
|
|
J. Erickson and T. Cline Key aspects of the primary sex determination mechanism are conserved across the genus Drosophila Development, January 8, 1998; 125(16): 3259 - 3268. [Abstract] [PDF]
|
|
|
|
|
|
D Perez-Mongiovi, P Chang, and E Houliston A propagated wave of MPF activation accompanies surface contraction waves at first mitosis in Xenopus J. Cell Sci., January 2, 1998; 111(3): 385 - 393. [Abstract] [PDF]
|
|
|
|
|
|
H White-Cooper, M. Schafer, L. Alphey, and M. Fuller Transcriptional and post-transcriptional control mechanisms coordinate the onset of spermatid differentiation with meiosis I in Drosophila Development, January 1, 1998; 125(1): 125 - 134. [Abstract] [PDF]
|
|
|
|
|
|
X Dong, K H Zavitz, B J Thomas, M Lin, S Campbell, and S L Zipursky Control of G1 in the developing Drosophila eye: rca1 regulates Cyclin A. Genes & Dev., January 1, 1997; 11(1): 94 - 105. [Abstract] [PDF]
|
|
|
|
 |
|
 B. A. Edgar and C. F. Lehner Developmental Control of Cell Cycle Regulators: A Fly's Perspective Science, December 6, 1996; 274(5293): 1646 - 1652. [Abstract] [Full Text]
|
|
|
|
|
|
B A Edgar and S A Datar Zygotic degradation of two maternal Cdc25 mRNAs terminates Drosophila's early cell cycle program. Genes & Dev., August 1, 1996; 10(15): 1966 - 1977. [Abstract] [PDF]
|
|
|
|
|
|
D K Pritchard and G Schubiger Activation of transcription in Drosophila embryos is a gradual process mediated by the nucleocytoplasmic ratio. Genes & Dev., May 1, 1996; 10(9): 1131 - 1142. [Abstract] [PDF]
|
|
|
|
|
|
L. Frenz and D. Glover A maternal requirement for glutamine synthetase I for the mitotic cycles of syncytial Drosophila embryos J. Cell Sci., January 11, 1996; 109(11): 2649 - 2660. [Abstract] [PDF]
|
|
|
|
|
|
J Creanor and J. Mitchison The kinetics of the B cyclin p56cdc13 and the phosphatase p80cdc25 during the cell cycle of the fission yeast Schizosaccharomyces pombe J. Cell Sci., January 6, 1996; 109(6): 1647 - 1653. [Abstract] [PDF]
|
|
|
|
|
|
S Hayashi A Cdc2 dependent checkpoint maintains diploidy in Drosophila Development, January 4, 1996; 122(4): 1051 - 1058. [Abstract] [PDF]
|
|
|
|
|
|
T. Lin, S Viswanathan, C Wood, P. Wilson, N Wolf, and M. Fuller Coordinate developmental control of the meiotic cell cycle and spermatid differentiation in Drosophila males Development, January 4, 1996; 122(4): 1331 - 1341. [Abstract] [PDF]
|
|
|
|
|
|
P. R. Mueller, T. R. Coleman, A. Kumagai, and W. G. Dunphy Myt1: A Membrane-Associated Inhibitory Kinase That Phosphorylates Cdc2 on Both Threonine-14 and Tyrosine-15 Science, October 6, 1995; 270(5233): 86 - 90. [Abstract] [PDF]
|
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