QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

This Article Full Text Full Text (PDF) Supplemental Research Data Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Liu, H. Articles by Shi, Y. Search for Related Content PubMed PubMed Citation Articles by Liu, H. Articles by Shi, Y. Related Content Related Article Social Bookmarking                   What's this?

Yin Yang 1 is a critical regulator of B-cell development

¹ Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115, USA; ² CBR Institute for Biomedical Research, Inc., Boston, Massachusetts 02115, USA; ³ Institute for Biology III, Albert-Ludwigs University of Freiburg and Max Planck Institute for Immunobiology, 79108 Freiburg, Germany; ⁴ Flow cytometry core facility, CBR Institute for Biomedical Research, Inc., Boston, Massachusetts 02115, USA; ⁵ Department of Immunology and Molecular Pathology, Division of Infection and Immunity, University College London, London W1T 4JF, United Kingdom

The role of the transcription factor Yin Yang 1 (YY1) in development is largely unknown. Here we show that specific ablation of YY1 in mouse B cells caused a defect in somatic rearrangement in the immunoglobulin heavy-chain (IgH) locus and a block in the progenitor-B-to-precursor-B-cell transition, which was partially rescued by a prerearranged IgH transgene. Three-dimensional DNA fluorescence in situ hybridization analysis revealed an important function for YY1 in IgH locus contraction, a process indispensable for distal V_H to D_HJ_H recombination. We provide evidence that YY1 binds the intronic Eiµ enhancer within the IgH locus, consistent with a direct role for YY1 in V_HD_HJ_H recombination. These findings identified YY1 as a critical regulator of early B-cell development.

[Keywords: YY1; immunoglobulin gene; B-cell development; V(D)J recombination]

Received January 9, 2007; revised version accepted March 22, 2007.

⁷ Endocrinology Division, Brigham and Women’s Hospital, Boston, MA 02115, USA;

⁸ Integrated Department of Immunology, National Jewish Medical and Research Center and University of Colorado Health Sciences Center, 1400 Jackson Street, Denver, CO 80206, USA.

⁹ Corresponding author.

E-MAIL yang_shi{at}hms.harvard.edu; FAX (617) 432-6687.

Supplemental material is available at http://www.genesdev.org.

Article is online at http://www.genesdev.org/cgi/doi/10.1101/gad.1529307

CiteULike    Connotea    Del.icio.us    Digg    Reddit    Technorati    What's this?

Related Article

YY1 helps to bring loose ends together

Kathryn Calame and Michael Atchison
Genes & Dev. 2007 21: 1145-1152. [Extract] [Full Text] [PDF]

This article has been cited by other articles:

				C.-R. Xu, L. Schaffer, S. R. Head, and A. J. Feeney Reciprocal patterns of methylation of H3K36 and H3K27 on proximal vs. distal IgVH genes are modulated by IL-7 and Pax5 PNAS, June 24, 2008; 105(25): 8685 - 8690. [Abstract] [Full Text] [PDF]

				E. Jacob, R. Hod-Dvorai, S. Schif-Zuck, and O. Avni Unconventional Association of the Polycomb Group Proteins with Cytokine Genes in Differentiated T Helper Cells J. Biol. Chem., May 9, 2008; 283(19): 13471 - 13481. [Abstract] [Full Text] [PDF]

				J. Kim and J. D. Kim In vivo YY1 knockdown effects on genomic imprinting Hum. Mol. Genet., February 1, 2008; 17(3): 391 - 401. [Abstract] [Full Text] [PDF]

				K. Calame and M. Atchison YY1 helps to bring loose ends together Genes & Dev., May 15, 2007; 21(10): 1145 - 1152. [Full Text] [PDF]