Errata for vol. 12, p. 480
Genes & Development 12: 480–490 (1998)
The Cdc7 protein kinase is required for origin firing during S phase
Kristin Bousset and John J.X. Diffley
Figure B of the above article was misprinted. The correct figure, in its entirety, appears below with its legend.
Cdc7 is not required for replication of a plasmid containing the early replication origin ARS305 after HU. (A) Outline of the experimental procedure (see text for details). (B) RM14-3a cells (cdc7-1) containing an ARS305 plasmid (p305.2) were treated as illustrated in A. Cells were grown in heavy medium and arrested with α factor (α). They were subsequently released into HU in light medium (HU) and then released from HU at either 24°C or 37°C for 3 hr as described in Materials and Methods. DNA was isolated and analyzed for replication. Replication of ARS305 on the plasmid was analyzed by DNA blot hybridization with a plasmid-specific probe (see Materials and Methods). The relative amounts of radioactivity are plotted against the fraction numbers. Locations of HH and HL DNA are indicated. Additionally, the HH peak as it appears in the G1 block is superimposed onto the other plots as a shaded line.
