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PERSPECTIVE

No exit strategy? No problem: APC inhibits -catenin inside the nucleus

Lineberger Comprehensive Cancer Center, Department of Biochemistry and Biophysics, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599, USA

The tumor suppressor adenomatous polyposis coli, APC, plays a critical role in regulating the growth, proliferation, and differentiation of cells in different tissues, including the colon. Heterozygous germline mutations in the APC gene predispose individuals to the development of colon cancer at a young age, and somatic mutations inactivating both APC alleles are associated with the majority of nonhereditary colon cancers. The best-established function of the APC protein is to negatively regulate the Wnt signaling pathway by antagonizing the function of -catenin, a dedicated transcriptional coactivator of Wnt target genes in the nucleus. The current model for the regulation of -catenin by APC rests on the ability of APC to bind with -catenin in the nucleus and export it out into the cytoplasm for targeted ubiquitination and proteasomal degradation. A report in the previous issue of Genes & Development Sierra et al. (2006) points to a separate mechanism for APC regulation of -catenin—repressing it on the chromatin.

	The Wnt signaling pathway

Top The Wnt signaling pathway Wnt signaling in the... APC exports beta-catenin out... beta-catenin recruits histone... APC counteracts beta-catenin... Exporting to cytoplasm and... Acknowledgments References

 
The Wnt gene family, whose name is derived from the Drosophila segment polarity gene Wingless and the murine proto-oncogene Int-1, encode secreted signaling proteins characterized by a cysteine-rich pattern instead of a discrete functional domain. The family is highly conserved throughout the animal kingdom, with five members in Caenorhabditis elegans, seven in flies, and 19 in mammals. The Wnt signaling pathway controls various cellular and biological processes, ranging from cell adhesion (Harris and Peifer 2005), stem cell self-renewal (Reya and Clevers 2005), and cancer development (Polakis 2000), to the differentiation of multiple cell lineages and development of various tissues (Cadigan and Nusse 1997). The critical importance of the Wnt signaling pathway in diverse processes was evident from early genetic and pathological studies. Loss of Drosophila Wingless gene function caused numerous developmental defects in embryonic and larval pattern formation and synaptic differentiation (Morata and Lawrence 1977), and oncogenic activation of murine Int-1 by retroviral insertion resulted in the development of mammary tumors (Nusse et al. 1984). Further support for the crucial role of the Wnt pathway comes from studies of its downstream targets. Homozygous deletion of Apc in mice caused embryonic lethality, and germline and somatic mutations in humans result in familial and sporadic colon cancer (Kinzler et al. 1991; Nishisho et al. 1991; Oshima et al. 1995). The null mutation of -catenin/Armadillo, an essential activator of the Wnt signaling pathway, caused early embryonic lethality at gastrulation in mice (Haegel et al. 1995) and segment polarity defects in Drosophila (Wieschaus and Riggleman 1987).

	Wnt signaling in the cytoplasm

Top The Wnt signaling pathway Wnt signaling in the... APC exports beta-catenin out... beta-catenin recruits histone... APC counteracts beta-catenin... Exporting to cytoplasm and... Acknowledgments References

 
In the canonical Wnt signaling pathway, engagement of a Wnt ligand with its receptor, a member of the Frizzled protein family, and a coreceptor of the LDL receptor-related protein (LRP) family, triggers the cytoplasmic tail of either Frizzled or LRP to interact with downstream components in the Wnt signaling pathway, including Dishevelled (Dsh) and Axin. A hallmark of Wnt pathway activation is the elevation in the cytoplasm and subsequent accumulation in the nucleus of -catenin/Armadillo, a protein with multiple functions that was first identified in flies as containing a patterning mutation with a phenotype similar to Wingless. -catenin was later found in vertebrates as a component of adherens junctions. It remains unclear how such an odd dual function—as a transcriptional activator in the nucleus to regulate Wnt signaling and as a membrane-bound form to mediate cell adhesion—evolved in one protein. It is intriguing to wonder whether the existence of two functions in one protein reflects an as yet unrecognized signaling of cytoskeletal stress to -catenin mediated transcription. In this brief perspective, we will focus on the regulation of -catenin in the context of Wnt signaling.

In the absence of Wnt signaling, APC and the scaffolding protein axin bind newly synthesized -catenin in the cytoplasm and facilitate sequential phosphorylation of -catenin in the so-called "destruction complex," first by casein kinase 1 (CK1) and then by glycogen synthase kinase 3 (GSK-3) on several residues including Ser33 and Ser37 (Amit et al. 2002; Liu et al. 2002; Yanagawa et al. 2002). Separately, APC protein is also phosphorylated, probably by the same GSK-3 and a different isoform of CK1, and phosphorylated APC has substantially higher affinity for binding with -catenin (Rubinfeld et al. 2001; Ha et al. 2004; Xing et al. 2004). Mechanistic details have yet to be worked out on the sequential order and functional relay within the destruction complex. Does phosphorylation of APC occur first and enhanced APC–-catenin binding then bring in -catenin for phosphorylation and prime it for subsequent ubiquitination? Or, does phosphorylation of APC follow -catenin phosphorylation, and the subsequent high-affinity association between APC and -catenin allows APC to move -catenin away from the destruction complex to a separate complex for ubiquitination (Xing et al. 2003)? Genetic studies from both human cancer patients and mutant mice support the critical, if not exclusive, functional relationship between APC and catenin. Gain-of-function mutations targeting -catenin and loss-of-function mutations targeting APC genes occur in a mutually exclusive manner in different tumors (Morin et al. 1997), supporting the notion that -catenin is a primary mediator of APC function and that APC is a major regulator of -catenin. Stabilizing mutations in the -catenin gene targeting the GSK-3 phosphorylation sites required for its degradation, Ser33, Thr44, and Ser45, were found in colorectal cancer cells (Morin et al. 1997), and deletion of exon 3 of mouse -catenin, which encodes 76 residues including these GSK-3 phosphorylation sites, caused adenomatous intestinal polyps (Harada et al. 1999).

How do APC, axin, CK1, GSK-3, and probably other proteins control the stability of -catenin in the cytoplasm? A critical link was made from genetic screens for recessive mutations affecting pattern formation in Drosophila. Mutation of Slimb/-TrCP, encoding a WD40 and F-box protein, produced a phenotype characteristic of ectopic Wnt activation and with high levels of -catenin protein (Jiang and Struhl 1998). Extensive biochemical studies have since been carried out and have led to a fairly good understanding of cytoplasmic ubiquitination of -catenin. Bridged by a small adaptor protein, Skp1, -catenin, following phosphorylation by this so-called degradation complex, is recruited to Cul1 by the F-box protein -TrCP for ubiquitination by the SCF^-TrCP E3 ligase (Kitagawa et al. 1999; Latres et al. 1999; Liu et al. 1999; Winston et al. 1999). In vitro, -TrCP/Slimb (also known FWD1 or HOS in mammals) forms a complex with -catenin in the Axin–GSK-3–APC degradation complex and promotes cytoplasmic degradation of -catenin. Subsequent structural analysis of Skp1 and -TrCP complexed with a phosphorylated -catenin peptide showed that Ser33 and Ser37 make direct contact with the -propeller of -TrCP (Wu et al. 2003). These detailed characterizations have made -catenin one of best-characterized SCF substrates, and have led to exploring the conditional knocking down of -catenin in APC-deficient colon cancer cells by inducible expression of a chimeric protein with the -catenin-binding domain of APC fused with -TrCP (Cong et al. 2003).

	APC exports -catenin out of the nucleus for cytoplasmic degradation

Top The Wnt signaling pathway Wnt signaling in the... APC exports beta-catenin out... beta-catenin recruits histone... APC counteracts beta-catenin... Exporting to cytoplasm and... Acknowledgments References

 
The current model of the control of -catenin by APC relies heavily on the intrinsic ability of APC to shuttle actively between the nucleus and the cytoplasm (Henderson 2000; Neufeld et al. 2000a, b; Rosin-Arbesfeld et al. 2000). APC encodes a large protein (2843 and 2303 residues for human APC and APC2, respectively) and normally distributes more in the cytoplasm than in the nucleus. Treatment of cells with leptomycin B (LMB), an inhibitor of CRM1-dependent nuclear export, accumulated APC protein in the nucleus. APC contains multiple nuclear export signals (NESs) that are recognized by CRM1, including at least two in the N-terminal region and three in the C-terminal region. The functionality of these NESs was demonstrated by the ability of each NES to individually exclude a reporter GFP fusion protein from the nucleus. Overexpression of wildtype APC in APC-deficient colon cancer cells enhanced nuclear export and degradation of -catenin. Conversely, mutations targeting NESs accumulated -catenin in the nucleus and increased its steady-state level, as in LMB-treated cells (Henderson 2000; Rosin-Arbesfeld et al. 2000), indicating that the ability of APC to undergo nuclear-cytoplasmic shuttling is critical in controlling -catenin degradation and that -catenin degradation occurs primarily, if not exclusively, in the cytoplasm.

There have been reports, however, that -catenin can shuttle between the cytoplasm and the nucleus independently of APC and CRM1 (Eleftheriou et al. 2001; Wiechens and Fagotto 2001). Whether and how the APC-independent nuclear-cytoplasmic shuttling of -catenin is coupled with Wnt signaling is yet to be established. The situation reminds one of the cytoplasmic degradation of the tumor suppressor p53 mediated by its principal inhibitor MDM2, an oncoprotein and a RING-type E3 ubiquitin ligase that functions both to ubiquitinate p53 as well as to export p53 to the cytoplasm for degradation (Zhang and Xiong 2001a). Like -catenin, nuclear export of p53 can be influenced by MDM2, but may also be independent of it. Mutations targeting the NES in MDM2 blocked MDM2 nuclear export and reduced MDM2-mediated p53 degradation (Roth et al. 1998). The p53 protein itself contains at least two functional NES, and DNA damage-induced phosphorylation on one serine residue (Ser15) within the N-terminal located NES impeded p53 export, contributing to nuclear accumulation and functional activation of p53 (Zhang and Xiong 2001b).

	-catenin recruits histone methyltransferases

Top The Wnt signaling pathway Wnt signaling in the... APC exports beta-catenin out... beta-catenin recruits histone... APC counteracts beta-catenin... Exporting to cytoplasm and... Acknowledgments References

 
Escaping from SCF^-TrCP-mediated ubiquitination and degradation following Wnt signaling, -catenin becomes stabilized and accumulates in the nucleus, leading to transcriptional activation of Wnt target genes by converting the HMG-box protein TCF/LEF repressor complex into a transcriptional activator complex. Numerous Wnt target genes have been reported and a number of proteins have been identified to interact with TCF (http://www.stanford.edu/~rnusse/wntwindow.html), reflecting the need to regulate diverse cellular and developmental functions of the Wnt signaling pathways. In the absence of Wnt signaling and -catenin, TCF forms a complex with Groucho, which brings in histone deacetylases (HDAC) to repress transcription activation (Cavallo et al. 1998; Roose et al. 1998; Chen et al. 1999). How -catenin switches TCF from a repressor into a transcriptional activator has been one of the focal points of current studies of Wnt signaling. The central region of -catenin contains twelve 40-amino-acid repetitive motifs known as armadillo repeats (ARM), which interact with an adaptor Bcl-9/Legless (Lgs), which connects -catenin to the PHD finger protein Pygopus (Pygo). Mutation in either the Lgs or Pygo gene in Drosophila resulted in a wingless-like phenotype, providing genetic support for their physiological function in Wnt signaling and showing the relevance of their interactions with -catenin. The Lgs and Pygo proteins form a single complex that has been suggested to anchor -catenin in the nucleus and present it to the TCF on chromatin (Townsley et al. 2004).

Expanding the stockpile of -catenin-interacting proteins is a region C-terminal to the ARM repeats, referred to as CTARM, that Kathy Jones’ group (Tutter et al. 2001) has previously shown to contain a strong activation domain necessary for -catenin-mediated transcription. The -catenin CTARM region, which includes ARM repeats 11 and 12 through the C terminus, functions as a chromatin-specific activation domain. Searching for proteins interacting with the -catenin CTARM domain led to two new findings reported by Kathy Jones’ group in a recent issue of Genes & Development (Sierra et al. 2006): that -catenin associates with the MLL1/MLL2 SET1-type histone methytransferase and promotes H3K4 trimethylation at a Wnt target gene, c-Myc, and that APC counteracts -catenin-mediated H3K4 methylation at Wnt target genes (Fig. 1). Trimethylation of H3K4 requires prior monoubiquitination of H2B (K123 in yeast and K120 in humans) (Zhang 2003; Ezhkova and Tansey 2004). Using pBRE (-catenin response element) plasmid chromatin templates assembled in vitro to measure RNA initiation by primer extension, the authors also showed that monoubiquitination, presumably of H2B K120, is essential for -catenin-dependent transcription. More vigorous in vivo examinations, such as CHIP assays, are needed to establish the functional dependency of -catenin-mediated transcription on H2B K120 ubiquitination. Extending previous work that Pygo and Lgs are required for retaining -catenin in the nucleus (Townsley et al. 2004), the authors showed that Pygo and Lgs directly bind with -catenin at the target gene. However, it is not clear whether Pygo and Bcl-9 are required for -catenin-mediated assembly of the coactivator complex. These findings further our understanding on the mechanistic role of -catenin on chromatin remodeling and in regulation of histone modification.

View larger version (85K): [in this window] [in a new window]   Figure 1. A model for transcriptional regulation of Wnt target genes by APC and -catenin. (A) When the Wnt signal is absent, a dual mechanism works to repress -catenin-mediated transcription. One is that APC directly associates with the TCF/LEF-binding site on the Wnt target genes and mediates the exchange between coactivator and corepressor complexes. The other is that APC exports -catenin from the nucleus to cytoplasm and transports it to the destruction complex, where it is phosphorylated and recognized by -TrCP, resulting in the polyubiquitination of -catenin by SCF-TrCP and subsequent degradation by the proteasome. (B) When Wnt ligand acts on its cell-surface receptor Frizzled, the phosphorylation of -catenin is inhibited, leading to its dissociation from the Axin-assembled destruction complex. Stabilized -catenin is accumulated to the nucleus and transiently binds to the transcription factor TCF/LEF, resulting in the activation of Wnt target genes.

	APC counteracts -catenin-mediated transcriptional activation on chromatin

Top The Wnt signaling pathway Wnt signaling in the... APC exports beta-catenin out... beta-catenin recruits histone... APC counteracts beta-catenin... Exporting to cytoplasm and... Acknowledgments References

-TrCP

The new results and the suggested model—APC counteracts -catenin-mediated transcriptional activation independent of nuclear export—is intriguing and brings up more questions. Could the newly observed interactions between APC and -TrCP with -catenin on chromatin be interpreted according to the current model? That is, do these interactions on chromatin represent a transient state during the dynamic APC-mediated -catenin exporting process in which APC comes to the chromatin to find -catenin, taking it off Wnt target gene promoters and exporting it out of the nucleus? What then is -TrCP doing on the chromatin? Could -TrCP target -catenin to SCF^-TrCP in the cytoplasm for polyubiquitination and degradation, but to a different ligase or E2 on the chromatin to cause nonproteolytic monoubiquitination and thus functional change of -catenin (or other proteins in the complex)? Do the two distinct regulatory mechanisms of -catenin regulation by APC functionally complement each other, with APC repressing -catenin on chromatin to rapidly and transiently inhibit Wnt signaling and exporting -catenin for cytoplasmic degradation to achieve a persistent or irreversible inhibition? Or do they work separately in different tissues or at varying times? If these two mechanisms work separately, how does APC distinguish when to export -catenin out of the nucleus for degradation and when to stay on the chromatin with -catenin to repress transcription? Given the intensity of research activity on the Wnt signaling pathway, there is good reason to believe that we will be learning much more soon.

	Exporting to cytoplasm and repressing on chromatin, a common dual mechanism?

Top The Wnt signaling pathway Wnt signaling in the... APC exports beta-catenin out... beta-catenin recruits histone... APC counteracts beta-catenin... Exporting to cytoplasm and... Acknowledgments References

 
Regulating the function of transcription factors by separating them in different cellular compartments or by active nuclear-cytoplasmic shutting is quite commonly used in many cellular pathways. Examples include nuclear-cytoplasmic shutting of the signal transducers and activators of transcription (STATs) and nuclear steroid hormone receptors (NRs) and the membrane anchoring of the sterol response element-binding protein (SREBP) that controls the promoters of sterol-regulated genes. What is interesting from the current study is that one protein, APC, may have evolved a dual mechanism to negatively regulate its target, -catenin, exporting it from the nucleus out to the cytoplasm for degradation and repressing -catenin-mediated transcription in situ on chromatin. This may not be unique for APC regulation of -catenin. Two other notable examples are the inhibition of NF-B by IB and p53 by MDM2. Like APC, IB can export its target, the NF-B protein, to the cytoplasm (Huang and Miyamoto 2001). Although first identified as cytoplasmic inhibitors of NF-B/Rel proteins, IB proteins also act in the nucleus and have been reported to associate with different corepressors and bind to the promoter region of the hes1 gene to repress its transcription (Aguilera et al. 2004). During normal (unstressed) cell growth, MDM2 keeps the p53 level, and thus its activity low, by promoting p53 export and subsequent degradation in the cytoplasm as APC regulation of -catenin in the absence of Wnt signaling. Genotoxic stresses activate p53 in part by blocking p53 and MDM2 nuclear export, and thus cytoplasmic degradation (Zhang and Xiong 2001a). Yet, blocking p53 and MDM2 nuclear export in the nucleus does not lead to p53 activation, suggesting that MDM2 can also repress p53 in the nucleus without causing p53 degradation, possibly by interfering with the basal level transcription machinery in situ on chromatin as suggested by an early study (Thut et al. 1997).

Most transcriptional repressors bind to their target activators on chromatin to inhibit transcription. It is not clear how many might have acquired the ability to undergo nuclear export and take their activators to the cytoplasm. What is the advantage to evolve such a dual mechanism for a repressor to regulate its transcriptional activator? Several can be speculated: Cytoplasmic degradation (or retention) can be inhibited quickly and simply by blocking nuclear export, allowing a rapid nuclear accumulation of transcription activators, and thus a prompt response to signaling events. Many transcriptional activators, especially those involved in different cellular pathways and regulating many different genes such as -catenin in Wnt signaling and p53 in checkpoint responses, are components of different and multisubunit complexes. Separating ubiquitination and the function of the transcriptional activator into two compartments would avoid inadvertent degradation of their interacting partners. While exporting -catenin out of the nucleus would inhibit the activation of most, if not all Wnt target genes, association of the APC–-catenin complex on chromatin could be limited to a subset of Wnt target genes, and therefore provide an additional level of specificity for Wnt signaling.

	Acknowledgments

Top The Wnt signaling pathway Wnt signaling in the... APC exports beta-catenin out... beta-catenin recruits histone... APC counteracts beta-catenin... Exporting to cytoplasm and... Acknowledgments References

 
We thank Al Baldwin, Kun-Liang Guan, Chad McCall, Bill Marzluff, Stuart Shumway, and Yi Zhang for suggestions and for reading the manuscript. This study is supported by NIH grants to Y.X.

	Footnotes

 
¹ Corresponding author.

E-MAIL yxiong{at}email.unc.edu; FAX (919) 966-8799.

Article and publication are at http://www.genesdev.org/cgi/doi/10.1101/gad.1413206

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