![[Feedback]](/icons/banner/feedbackACT.gif){kind=icon}
![[For Subscribers]](/icons/banner/subscriberACT.gif){kind=icon}
![[Archive]](/icons/banner/archiveACT.gif){kind=icon}
![[Search]](/icons/banner/searchACT.gif){kind=icon}
|
|
|
|
|
||
| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
RESEARCH PAPER
-catenin signaling is sufficient and necessary for synovial joint formation
Genetic Disease Research Branch, National Human Genome Research Institute, Bethesda, Maryland 20892, USA
A critical step in skeletal morphogenesis is the formation of synovial
joints, which define the relative size of discrete skeletal elements and are
required for the mobility of vertebrates. We have found that several
Wnt genes, including Wnt4, Wnt14, and Wnt16, were
expressed in overlapping and complementary patterns in the developing synovial
joints, where 
-catenin protein levels and transcription activity were
up-regulated. Removal of -catenin early in mesenchymal
progenitor cells promoted chondrocyte differentiation and blocked the activity
of Wnt14 in joint formation. Ectopic expression of an activated form
of -catenin or Wnt14 in early differentiating
chondrocytes induced ectopic joint formation both morphologically and
molecularly. In contrast, genetic removal of -catenin in
chondrocytes led to joint fusion. These results demonstrate that the
Wnt/-catenin signaling pathway is necessary and sufficient to induce
early steps of synovial joint formation. Wnt4, Wnt14, and
Wnt16 may play redundant roles in synovial joint induction by
signaling through the -catenin-mediated canonical Wnt pathway.
Received June 10, 2004; revised version accepted August 9, 2004.
Article published online ahead of print. Article and publication date are at http://www.genesdev.org/cgi/doi/10.1101/gad.1230704.
1 These authors contributed equally to this work.
2 Present address: Department of Biochemistry and State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 210093, P. R. China
3 Corresponding author.
E-MAIL
yyang{at}nhgri.nih.gov;
FAX (301) 402-2170.
CiteULike 
Connotea 
Del.icio.us 
Digg 
Reddit 
Technorati What's this?
This article has been cited by other articles:
|
|
 |
|
  M. L. Sohaskey, J. Yu, M. A. Diaz, A. H. Plaas, and R. M. Harland JAWS coordinates chondrogenesis and synovial joint positioning Development, July 1, 2008; 135(13): 2215 - 2220. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Raz, S. Stricker, E. Gazzerro, J. L. Clor, F. Witte, H. Nistala, S. Zabski, R. C. Pereira, L. Stadmeyer, X. Wang, et al. The mutation ROR2W749X, linked to human BDB, is a recessive mutation in the mouse, causing brachydactyly, mediating patterning of joints and modeling recessive Robinow syndrome Development, May 1, 2008; 135(9): 1713 - 1723. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. F. Day and Y. Yang Wnt and Hedgehog Signaling Pathways in Bone Development J. Bone Joint Surg. Am., February 1, 2008; 90(Supplement_1): 19 - 24. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. PROVOT and E. SCHIPANI Fetal Growth Plate: A Developmental Model of Cellular Adaptation to Hypoxia Ann. N.Y. Acad. Sci., November 1, 2007; 1117(1): 26 - 39. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Masuya, K. Nishida, T. Furuichi, H. Toki, G. Nishimura, H. Kawabata, H. Yokoyama, A. Yoshida, S. Tominaga, J. Nagano, et al. A novel dominant-negative mutation in Gdf5 generated by ENU mutagenesis impairs joint formation and causes osteoarthritis in mice Hum. Mol. Genet., October 1, 2007; 16(19): 2366 - 2375. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Provot, D. Zinyk, Y. Gunes, R. Kathri, Q. Le, H. M. Kronenberg, R. S. Johnson, M. T. Longaker, A. J. Giaccia, and E. Schipani Hif-1{alpha} regulates differentiation of limb bud mesenchyme and joint development J. Cell Biol., May 7, 2007; 177(3): 451 - 464. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. V. Semenov and X. He LRP5 Mutations Linked to High Bone Mass Diseases Cause Reduced LRP5 Binding and Inhibition by SOST J. Biol. Chem., December 15, 2006; 281(50): 38276 - 38284. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Zhang and M. J. Cohn Hagfish and lancelet fibrillar collagens reveal that type II collagen-based cartilage evolved in stem vertebrates PNAS, November 7, 2006; 103(45): 16829 - 16833. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. K. Mak, M.-H. Chen, T. F. Day, P.-T. Chuang, and Y. Yang Wnt/{beta}-catenin signaling interacts differentially with Ihh signaling in controlling endochondral bone and synovial joint formation Development, September 15, 2006; 133(18): 3695 - 3707. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Spater, T. P. Hill, R. J. O'Sullivan, M. Gruber, D. A. Conner, and C. Hartmann Wnt9a signaling is required for joint integrity and regulation of Ihh during chondrogenesis Development, August 1, 2006; 133(15): 3039 - 3049. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Torban, A. Dziarmaga, D. Iglesias, L. L. Chu, T. Vassilieva, M. Little, M. Eccles, M. Discenza, J. Pelletier, and P. Goodyer PAX2 Activates WNT4 Expression during Mammalian Kidney Development J. Biol. Chem., May 5, 2006; 281(18): 12705 - 12712. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. PACIFICI, E. KOYAMA, Y. SHIBUKAWA, C. WU, Y. TAMAMURA, M. ENOMOTO-IWAMOTO, and M. IWAMOTO Cellular and Molecular Mechanisms of Synovial Joint and Articular Cartilage Formation Ann. N.Y. Acad. Sci., April 1, 2006; 1068(1): 74 - 86. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
W. Satoh, T. Gotoh, Y. Tsunematsu, S. Aizawa, and A. Shimono Sfrp1 and Sfrp2 regulate anteroposterior axis elongation and somite segmentation during mouse embryogenesis Development, March 15, 2006; 133(6): 989 - 999. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. I. Reinhold, R. M. Kapadia, Z. Liao, and M. C. Naski The Wnt-inducible Transcription Factor Twist1 Inhibits Chondrogenesis J. Biol. Chem., January 20, 2006; 281(3): 1381 - 1388. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Tamamura, T. Otani, N. Kanatani, E. Koyama, J. Kitagaki, T. Komori, Y. Yamada, F. Costantini, S. Wakisaka, M. Pacifici, et al. Developmental Regulation of Wnt/{beta}-Catenin Signals Is Required for Growth Plate Assembly, Cartilage Integrity, and Endochondral Ossification J. Biol. Chem., May 13, 2005; 280(19): 19185 - 19195. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Kobayashi and H. Kronenberg Minireview: Transcriptional Regulation in Development of Bone Endocrinology, March 1, 2005; 146(3): 1012 - 1017. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. Seeman and G. J. Strewler Clinical and Basic Research Papers - September and October 2004 Selections IBMS BoneKEy, November 1, 2004; 1(11): 1 - 5. [Full Text] [PDF]
|
|
|
|
|
|
E. Seeman and G. J. Strewler Clinical and Basic Research Papers - August 2004 Selections IBMS BoneKEy, October 1, 2004; 1(10): 1 - 3. [Full Text] [PDF]
|
|
