Genes and Development

Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
QUICK SEARCH:   [advanced]


     

GENES & DEVELOPMENT 21:385-390, 2007
©2007 by Cold Spring Harbor Laboratory Press; ISSN 0890-9369/ $5.00
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Supplemental Research Data
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zeitlinger, J.
Right arrow Articles by Levine, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zeitlinger, J.
Right arrow Articles by Levine, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us   Add to Digg   Add to Reddit   Add to Technorati  
What's this?

RESEARCH COMMUNICATION

Whole-genome ChIP–chip analysis of Dorsal, Twist, and Snail suggests integration of diverse patterning processes in the Drosophila embryo

Julia Zeitlinger1, Robert P. Zinzen2, Alexander Stark3,4, Manolis Kellis3,4, Hailan Zhang5, Richard A. Young1,6, and Michael Levine2,7

1 Whitehead Institute for Biomedical Research, Nine Cambridge Center, Cambridge, Massachusetts 02142, USA; 2 Department of Molecular Cell Biology, Center for Integrative Genomics, University of California, Berkeley, California 94720, USA; 3 Broad Institute of Massachusetts Institute of Technology and Harvard, Cambridge, Massachusetts 02141, USA; 4 Computer Science and Artificial Intelligence Laboratory, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA; 5 Department of Medicine, Mount Sinai School of Medicine, New York, New York 10029, USA; 6 Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts 02139, USA

Genetic studies have identified numerous sequence-specific transcription factors that control development, yet little is known about their in vivo distribution across animal genomes. We determined the genome-wide occupancy of the dorsoventral (DV) determinants Dorsal, Twist, and Snail in the Drosophila embryo using chromatin immunoprecipitation coupled with microarray analysis (ChIP–chip). The in vivo binding of these proteins correlate tightly with the limits of known enhancers. Our analysis predicts substantially more target genes than previous estimates, and includes Dpp signaling components and anteroposterior (AP) segmentation determinants. Thus, the ChIP–chip data uncover a much larger than expected regulatory network, which integrates diverse patterning processes during development.

[Keywords: Dorsoventral patterning; segmentation; gene regulatory networks; ChIP–chip]

revised version accepted January 11, 2007.

7 Corresponding author.

E-MAIL mlevine{at}berkeley.edu; FAX (510) 643-5785.

Article is online at http://www.genesdev.org/cgi/doi/10.1101/gad.1509607


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us   Add to Digg Digg   Add to Reddit Reddit   Add to Technorati Technorati    What's this?


This article has been cited by other articles:


Home page
 Proc. Natl. Acad. Sci. USAHome page
D. A. Hendrix, J.-W. Hong, J. Zeitlinger, D. S. Rokhsar, and M. S. Levine
Promoter elements associated with RNA Pol II stalling in the Drosophila embryo
PNAS, June 3, 2008; 105(22): 7762 - 7767.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
M. B. Noyes, X. Meng, A. Wakabayashi, S. Sinha, M. H. Brodsky, and S. A. Wolfe
A systematic characterization of factors that regulate Drosophila segmentation via a bacterial one-hybrid system
Nucleic Acids Res., May 1, 2008; 36(8): 2547 - 2560.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
C. A. Martinez and D. N. Arnosti
Spreading of a Corepressor Linked to Action of Long-Range Repressor Hairy
Mol. Cell. Biol., April 15, 2008; 28(8): 2792 - 2802.
[Abstract] [Full Text] [PDF]

Home page
 Genome Res.Home page
P. Kheradpour, A. Stark, S. Roy, and M. Kellis
Reliable prediction of regulator targets using 12 Drosophila genomes
Genome Res., December 1, 2007; 17(12): 1919 - 1931.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
W. Zhong and P. W. Sternberg
Automated data integration for developmental biological research
Development, September 15, 2007; 134(18): 3227 - 3238.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
C. D. Brown, D. S. Johnson, and A. Sidow
Functional Architecture and Evolution of Transcriptional Elements That Drive Gene Coexpression
Science, September 14, 2007; 317(5844): 1557 - 1560.
[Abstract] [Full Text] [PDF]

Home page
 Brief Funct Genomic ProteomicHome page
T. D. Southall and A. H. Brand
Chromatin profiling in model organisms
Brief Funct Genomic Proteomic, July 24, 2007; (2007) elm013v1.
[Abstract] [Full Text] [PDF]


Home Help [Feedback] [For Subscribers] [Archive] [Search] [Contents]
Genome Res. Learn. Mem.
Protein Science RNA Genes Dev.
Copyright © 2007 by Cold Spring Harbor Laboratory Press.