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GENES & DEVELOPMENT 21:3238-3243, 2007
©2007 by Cold Spring Harbor Laboratory Press; ISSN 0890-9369/ $5.00
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RESEARCH COMMUNICATION

A role for microRNAs in maintenance of mouse mammary epithelial progenitor cells

Ingrid Ibarra1,2, Yaniv Erlich1, Senthil K. Muthuswamy1, Ravi Sachidanandam1, and Gregory J. Hannon1,3

1 Watson School of Biological Sciences, Howard Hughes Medical Institute, Cold Spring Harbor Laboratory, Cold Spring Harbor, New York 11724, USA; 2 Program in Genetics, Stony Brook University, Stony Brook, New York 11794, USA

microRNA (miRNA) expression profiles are often characteristic of specific cell types. The mouse mammary epithelial cell line, Comma-Dβ, contains a population of self-renewing progenitor cells that can reconstitute the mammary gland. We purified this population and determined its miRNA signature. Several microRNAs, including miR-205 and miR-22, are highly expressed in mammary progenitor cells, while others, including let-7 and miR-93, are depleted. Let-7 sensors can be used to prospectively enrich self-renewing populations, and enforced let-7 expression induces loss of self-renewing cells from mixed cultures.

[Keywords: RNAi; microRNA; stem cell]]

Received September 17, 2007; revised version accepted October 30, 2007.

3 Corresponding author.

E-MAIL hannon{at}cshl.edu; FAX (516) 367-8874.

Supplemental material is available at http://www.genesdev.org.

Article is online at http://www.genesdev.org/cgi/doi/10.1101/gad.1616307


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