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GENES & DEVELOPMENT 22:1190-1204, 2008
©2008 by Cold Spring Harbor Laboratory Press; ISSN 0890-9369/ $5.00
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Actively transcribed rRNA genes in S. cerevisiae are organized in a specialized chromatin associated with the high-mobility group protein Hmo1 and are largely devoid of histone molecules

Katharina Merz1, Maria Hondele2, Hannah Goetze, Katharina Gmelch, Ulrike Stoeckl, and Joachim Griesenbeck3

Universitaet Regensburg, Institut für Biochemie, Genetik und Mikrobiologie, 93053 Regensburg, Germany

Synthesis of ribosomal RNAs (rRNAs) is the major transcriptional event in proliferating cells. In eukaryotes, ribosomal DNA (rDNA) is transcribed by RNA polymerase I from a multicopy locus coexisting in at least two different chromatin states. This heterogeneity of rDNA chromatin has been an obstacle to defining its molecular composition. We developed an approach to analyze differential protein association with each of the two rDNA chromatin states in vivo in the yeast Saccharomyces cerevisiae. We demonstrate that actively transcribed rRNA genes are largely devoid of histone molecules, but instead associate with the high-mobility group protein Hmo1.

[Keywords: Chromatin; transcription; ribosomal DNA]]

Received December 11, 2007; revised version accepted February 29, 2008.

1 Present addresses: Helmholtz Zentrum München-German Research Center for Environmental Health (GmbH), 85764 Neuherberg, Germany;

2 European Molecular Biology Laboratory (EMBL) Heidelberg, 69117 Heidelberg, Germany.

3 Corresponding author.

E-MAIL joachim.griesenbeck{at}vkl.uni-regensburg.de; FAX 0941-943-2474.

Supplemental material is available at http://www.genesdev.org.

Article is online at http://www.genesdev.org/cgi/doi/10.1101/gad.466908.


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