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Transinduction of intergenic transcription in the human
and mouse
-globin locus control region (LCR). (Top) RNA
FISH analysis showing colocalization of transcription from a
transfected
-globin-containing plasmid (green) with transcripts
from the endogenous LCR (red) in HeLa cells. (Center) DNA
FISH analysis showing a transfected (left) or nontransfected
(right) DAPI-stained HeLa nucleus (blue) with the
transducing plasmid DNA (red) and chromosomal
-globin locus
(green) associated. (Bottom) RNA FISH analysis showing a colocalization of mouse LCR (red) and mouse
-major transcript (green) in a transgenic mouse fetal liver cell that contains a single
copy of the human
-globin locus. (For details, see Ashe et al., p.
2494.)