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Single-minded (Sim) controls CNS midline cell specification. Shown is a confocal immunofluorescence image of a Drosophila embryo just after gastrulation, stained with antibodies against Sim (green) and costained with propidium iodide (red) to visualize the DNA. At this stage, Sim is restricted to the developing CNS midline cells. (For details, see Crews, p. 607.)