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SCF ubiquitin ligase complexes localize to the centrosome. Shown here are fluorescence microscopic images of NIH-3T3 cells stained with antibodies to Skp1, a component of SCF ubiquitin ligases (red) and
-tubulin (green), and counterstained with Hoechst, which visualizes the DNA. The top right cell is in metaphase; the bottom left two cells are in telophase. (For details, see Freed et al., p. 2242.)