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FACKEL encodes a sterol C-14 reductase important in Arabidopsis embryogenesis. Shown here is an Arabidopsis fk-X224 mutant seedling that exhibits a short, thickened root, short hypocotyl, and misshaped cotyledons. The structural formula for 5
-ergosta-8,14-dien-3
-ol, an unreduced
8,14 sterol, is shown. FK mutant seedlings accumulate unreduced biosynthetic intermediates (green), indicating a block at the sterol C-14 reductase step. (For details, see Schrick et al., p. 1471; see also Jang et al., p. 1485.)