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Notch signaling is required for follicle cells to switch from the mitotic cell cycle to the endocycle during Drosophila oogenesis. Shown here is a surface view of a stage 10B Drosophila egg chamber containing a large presenilin mutant clone stained for
-Tubulin (magenta) and phospho-histone-H3 (blue). The green staining at the left of the image corresponds to GFP-Staufen in nurse cells. Phospho-histone-H3 staining shows that mutant follicle cells remain mitotically active, and are unable to switch to the endocycle at mid-oogenesis. (For details, see López-Scheir et al., p. 1393.)