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Ddc1 localizes to a single subnuclear focus following an endonucleolytic break. Shown here are S. cerevisiae cells expressing a Ddc1-GFP fusion protein. Induction of a double-stranded DNA break was generated by the site-specific HO endonuclease. Following HO exposure, Ddc1-GFP is recruited from its diffuse nuclear localization to form a single subnuclear focus at the site of DNA damage. Ddc1 is a conserved protein which contains homology to the replication factor PCNA and is required for DNA damage checkpoint-dependent cell cycle arrest. (For details, see Melo et al., p. 2809.)