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Drosophila amphiphysin is localized on muscle T-tubules, and is required for T-tubule organization. Shown here is an immunofluorescence analysis of a wild-type adult indirect flight muscle using antibodies directed against amphiphysin (green) and F-actin (red). The amphiphysin-containing T-tubules course intimately around and between the actin-containing myofibrils, thereby conducting depolarization from the plasma membrane to intracellular calcium stores upon muscle excitation. The novel role of amphiphysin in T-tubule organization is unexpected on the basis of its best known role in vertebrate endocytosis. (For details, see Razzaq et al., p. 2967.)