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Krox-20 ectopic expression in the hindbrain leads to the activation of EphA4 and the repression of Hoxb1. Shown here is an in situ hybridization analysis of a flat-mounted chick hindbrain following electroporation with a Krox-20 expression plasmid. The hindbrain was hybridized with EphA4 (red) and Hoxb1 (purple) probes. The right side was not electroporated; it exhibits the normal segmented pattern of EphA4 and Hoxb1 expression. The left side was electroporated with the Krox-20 plasmid, leading to major distortions in EphA4 and Hoxb1 expression. (For details, see Giudicelli et al., p. 567.)