
The widely conserved bacterial protein ZapA promotes Z-ring formation during cytokinesis. Shown here are Bacillus subtilis cells expressing a ZapA-Green Fluorescent Protein fusion construct (GFP-ZapA, green) that were treated with the fluorescent membrane stain TMA-DPH (red). GFP-ZapA localizes at the mid cell position as a band extending along the short axis of the cell, marking the site of future septum formation. Please note that the cover represents a composite of the repeated image of the same field of cells. (For details, see Gueiros-Filho and Losick, p. 2544.)