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Cover Ras-mediated phosphorylation of the transcription factor Net induces angiogenesis. Shown here is an immunofluorescence analysis of NIH3T3 fibroblast cells that were cotransfected with expression vectors for Net and Ras-V12. The cells were stained with antibodies directed against VEGF (red) and phosphorylated Net (P-Net; green). Transfected cells fluoresce brightly, with VEGF (red) in the cytoplasm and P-Net (green) in the nucleus. (For details, see Zheng et al., p. 2283.)