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Cover The differentiation of myoblasts to myotubes involves a switch in the composition of the core transcription machinery, involving the replacement of the canonical holo-TFIID complex by a novel TAF3/TRF3 complex. Shown here (from left to right) are immunofluorescence profiles of myotubes stained with antibodies (red) directed against TAF4, TAF3, TBP, and TRF3. DAPI (blue) and Phalloidin (green) staining were used to visualize DNA and the actin cytoskeleton, respectively. In contrast to the strong TAF3 and TRF3 nuclear stain in these cells, the myotubes are devoid of TAF4 and TBP. The TAF3/TRF3 complex is required for the regulation of the transcription program in terminally differentiated skeletal muscle cell types. (For details, see Deato and Tjian, p. 2137.)