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Cover Loss of histone H2B ubiquitylation is associated with abnormal nuclear morphology in fission yeast. Shown here is an immunofluorescence analysis of a htb1-K119R mutant cell, in which the conserved ubiquitylation site in H2B has been removed. Staining was carried out using antibodies directed against the nuclear pore (green) and the DNA-binding dye DAPI (blue). In contrast to the smooth, crescent-shaped DAPI staining pattern seen in wild-type cells, the DAPI-stained region of the mutant nucleus is fragmented, indicating a defect in global nuclear architecture. (For details, see Tanny et al., p. 835.)