Cover image

Cover Neural rosette cells (R-NSCs) are a novel neural stem cell type that display broad differentiation potential toward both CNS and PNS cell fates. Human embryonic stem cell-derived clonal rosette-stage cultures self-renew at the R-NSC state and give rise to more restricted NSC populations (NSCFGF/EGF). Shown here is a nonconfocal image of an immunofluorescence analysis of clonally expanded, GFP-tagged R-NSCs (green). GFP-tagged R-NSCs give rise to GFP⁺ cells that retain epithelial organization and express the R-NSC nuclear marker PLZF (red), as well as NSCFGF/EGF cells, which lose epithelial organization and PLZF expression and acquire expression of the NSCFGF/EGF cytoplasmic marker S100B (blue). (For details, see Elkabetz et al., p. 152.)